Active Vascular Endothelial Growth Factor 121 (VEGF121)
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 等电点Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg (保证有货 万一缺货 免费重做)
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- Gene sequencing
- Figure. Western Blot; Sample: Recombinant VEGF121, Human.
- 通过ISO 9001、ISO 13485质量体系认证
VEGFA (Vascular endothelial growth factor A) is a growth factor and can be cleaved into several isoforms, including VEGF121. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It is accepted that the VEGF121 isoform stimulates the proliferation of vein endothelial cells. Thus, proliferation assay of recombinant human VEGF121 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of VEGF121. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with VEGF121 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant VEGF121 for 48h. The result was shown in Figure 2. It was obvious that VEGF121 significantly increased cell viability of ECV-304 cells.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|RPB851Hu01||血管内皮生长因子121(VEGF121)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB851Hu02||血管内皮生长因子121(VEGF121)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APB851Hu01||血管内皮生长因子121(VEGF121)活性蛋白||Cell culture; Activity Assays.|
|PAB851Hu01||血管内皮生长因子121(VEGF121)多克隆抗体||WB; IHC; ICC; IP.|
|PAB851Hu02||血管内皮生长因子121(VEGF121)多克隆抗体||WB; IHC; ICC; IP.|
|LAB851Hu71||血管内皮生长因子121(VEGF121)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAB851Hu21||血管内皮生长因子121(VEGF121)单克隆抗体||WB; IHC; ICC; IP.|
|SEB851Hu||血管内皮生长因子121(VEGF121)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSB851Hu01||血管内皮生长因子121(VEGF121)抗体对||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSB851Hu01||血管内皮生长因子121(VEGF121)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It (ELISA Kit) Yourself".|
|Oncogene||Elevated PLGF contributes to small-cell lung cancer brain metastasis [PubMed: 22797069]|