血管内皮生长因子C(VEGFC)活性蛋白

Active Vascular Endothelial Growth Factor C (VEGFC)

VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein

  • 血管内皮生长因子C(VEGFC)活性蛋白 产品包装(模拟)
  • 血管内皮生长因子C(VEGFC)活性蛋白 产品包装(模拟)
  • APA145Hu01.jpg SDS-PAGE图
  • 血管内皮生长因子C(VEGFC)活性蛋白 Figure. Western Blot; Sample: Recombinant VEGFC, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. The dose-effect curve of VEGFC was shown in Figure 1. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339µg/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA145Hu01 血管内皮生长因子C(VEGFC)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA145Hu01 血管内皮生长因子C(VEGFC)活性蛋白 Cell culture; Activity Assays.
PAA145Hu01 血管内皮生长因子C(VEGFC)多克隆抗体 WB; IHC; ICC; IP.
MAA145Hu22 血管内皮生长因子C(VEGFC)单克隆抗体 WB; IHC; ICC; IP.
SEA145Hu 血管内皮生长因子C(VEGFC)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA145Hu 血管内皮生长因子C(VEGFC)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA145Hu01 血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Cellular Biochemistry Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma [PubMed: 19241443]
genetics and molecular research Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stages [PubMed: 26125824]
Experimental and Therapeutic Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia [PubMed: 26622498]
Am J Pathol IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages [PubMed: 26608451]
PLoS One. Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells. [pubmed:27383632]
Oncology Letters RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo. [pubmed:27895746]
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