血管内皮生长因子受体2(VEGFR2)活性蛋白

Active Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)

CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1

  • 血管内皮生长因子受体2(VEGFR2)活性蛋白 产品包装(模拟)
  • 血管内皮生长因子受体2(VEGFR2)活性蛋白 产品包装(模拟)
  • 血管内皮生长因子受体2(VEGFR2)活性蛋白 Figure. Gene Sequencing (Extract)
  • APB367Hu61.png Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. Cell proliferation of ECV-304 cells inhibit by VEGFR2.
Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) also known as kinase insert domain receptor acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. VEGFR2 functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. Regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. To test the effect on inhibit the VEGF-dependent proliferation of endothelium cells, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1μg/mL Vascular Endothelial Growth Factor C (VEGFC) and various concentrations of recombinant human VEGFR2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of ECV-304 cells after incubation with VEGFR2 for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant VEGFR2 for 96h. The result was shown in Figure 2. It was obvious that VEGFR2 significantly inhibit cell viability of ECV-304.
(A) ECV-304 cells cultured in DMEM, stimulated with 10ng/mL VEGFR2 for 96h;
(B) Unstimulated ECV-304 cells cultured in DMEM for 96h.

Figure. VEGFR2 inhibit VEGF-dependent proliferation of ECV-304 cells.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB367Hu61 血管内皮生长因子受体2(VEGFR2)活性蛋白 Cell culture; Activity Assays.
EPB367Hu61 血管内皮生长因子受体2(VEGFR2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB367Hu01 血管内皮生长因子受体2(VEGFR2)活性蛋白 Cell culture; Activity Assays.
RPB367Hu01 血管内皮生长因子受体2(VEGFR2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB367Hu01 血管内皮生长因子受体2(VEGFR2)多克隆抗体 WB; IHC; ICC; IP.
LAB367Hu81 血管内皮生长因子受体2(VEGFR2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB367Hu71 血管内皮生长因子受体2(VEGFR2)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB367Hu22 血管内皮生长因子受体2(VEGFR2)单克隆抗体 WB; IHC; ICC; IP.
MAB367Hu21 血管内皮生长因子受体2(VEGFR2)单克隆抗体 WB; IHC; ICC; IP.
HEB367Hu 血管内皮生长因子受体2(VEGFR2)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB367Hu 血管内皮生长因子受体2(VEGFR2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB367Hu01 血管内皮生长因子受体2(VEGFR2)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
APMIS Peritumoral brain edema in angiomatous supratentorial meningiomas: an investigation of the vascular endothelial growth factor A pathway [PubMed: 23398358]
Acta Biomater Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470]
Acta Biomaterialia Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470]
Journal of Cellular Physiology A Human 3D In Vitro Model to Assess the Relationship Between Osteoporosis and Disseminationto Bone of Breast Cancer Tumor Cells. [pubmed:27925188]
Regenerative Biomaterials The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic [10.1093/rb/rbx028]
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