玻连蛋白(VTN)活性蛋白

Active Vitronectin (VTN)

V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

  • 玻连蛋白(VTN)活性蛋白 产品包装(模拟)
  • 玻连蛋白(VTN)活性蛋白 产品包装(模拟)
  • APB041Ra01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Vitronectin (VTN ) is a glycoprotein of the hemopexin family which is abundantly found in serum, the extracellular matrix and bone. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. VTN can binds to integrin alpha-V beta-3 and thus promotes cell adhesion and spreading. It also binds to plasminogen activator inhibitor-1 (PAI-1) and serves to regulate proteolysis initiated by plasminogen activation. Besides, Insulin Like Growth Factor Binding Protein 5 (IGFBP5) has been identified as an interactor of VTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant Rat VTN and recombinant Rat IGFBP5. Briefly, VTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to IGFBP5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of VTN and IGFBP5 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB041Ra01 玻连蛋白(VTN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB041Ra01 玻连蛋白(VTN)活性蛋白 Cell culture; Activity Assays.
PAB041Ra01 玻连蛋白(VTN)多克隆抗体 WB; IHC; ICC; IP.
MAB041Ra21 玻连蛋白(VTN)单克隆抗体 WB; IHC; ICC; IP.
SEB041Ra 玻连蛋白(VTN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB041Ra 玻连蛋白(VTN)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSB041Ra01 玻连蛋白(VTN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563]
PLoS ONE Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance [PubMed: PMC3754973]
Liver International Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE [Pubmed: 23944848]
Analytical Biochemistry A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots [Pubmed:29154788]
Journal of Materials Chemistry B Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs [Doi: 10.1039/C8TB02283F ]
JOURNAL OF NEPHROLOGY Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts [33275196]
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