无翅型MMTV整合位点家族成员5A(WNT5A)活性蛋白

Active Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

  • 无翅型MMTV整合位点家族成员5A(WNT5A)活性蛋白 产品包装(模拟)
  • 无翅型MMTV整合位点家族成员5A(WNT5A)活性蛋白 产品包装(模拟)
  • 无翅型MMTV整合位点家族成员5A(WNT5A)活性蛋白 Figure. Gene Sequencing (Extract)
  • APP549Ra01.jpg Figure. SDS-PAGE
  • 无翅型MMTV整合位点家族成员5A(WNT5A)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of WNT5A with WIF1.
Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) is a ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Besides, WNT Inhibitory Factor 1 (WIF1) has been identified as an interactor of WNT5A, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat WNT5A and recombinant rat WIF1. Briefly, WNT5A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to WIF1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-WNT5A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of WNT5A and WIF1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)活性蛋白 Cell culture; Activity Assays.
PAP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)多克隆抗体 WB; IHC; ICC; IP.
MAP549Ra21 无翅型MMTV整合位点家族成员5A(WNT5A)单克隆抗体 WB; IHC; ICC; IP.
SEP549Ra 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Liver International Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [Pubmed:25488180]
Journal of Physiology and Biochemistry Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells [PubMed: 26547443]
Liver Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [PubMed: 25488180]
Transl Res Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures [PubMed: 26163991]
不确定杂志名 Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an … [servlets:MCRFileNodeServlet]
Life Sciences Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618]
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