抗抗增殖细胞核抗原(PCNA)单克隆抗体

Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody

Cyclin

  • 编号CAA591Mi22
  • 物种Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠), Canis familiaris; Canine (Dog,犬) 相同的名称,不同的物种。
  • 纯化方式蛋白A + 蛋白G亲和纯化
  • 标记物无标记物
  • 缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
  • 性状液体
  • 浓度1mg/mL
  • 且适物种Hamster (Chinese hamster,仓鼠), Sus scrofa; Porcine (Pig,猪), Bos taurus; Bovine (Cattle,牛), Capra hircus; Caprine (Goat,山羊), Ovis aries; Ovine (Sheep,绵羊), Chicken (Gallus,鸡)
  • 应用Loading Control of WB
  • 下载 英文说明书   中文说明书
  • 规格 20µl100µl 200µl 1ml 10ml
  • 价格 ¥ 716 ¥ 1672 ¥ 2388 ¥ 5970 ¥ 23880
  • 欲了解实际交易价格和更多情况,请与当地经销商联系!
  • 抗抗增殖细胞核抗原(PCNA)单克隆抗体 产品包装(模拟)
  • 抗抗增殖细胞核抗原(PCNA)单克隆抗体 产品包装(模拟)
  • CAA591Mi22.jpg WB图
  • 抗抗增殖细胞核抗原(PCNA)单克隆抗体 Western Blot; Sample: Lane1: A549 cell lysate; Lane2: 293T cell lysate; Lane3: A875 cell lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate; Lane6: K562 cell lysate; Lane7: MCF7 cell lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗抗增殖细胞核抗原(PCNA)单克隆抗体 Western Blot; Sample: Lane1: U87MG cell lysate; Lane2: SGC7901 cell lysate; Lane3: ECV304 cell lysate; Lane4: PC3 cell lysate; Lane5: A375 cell lysate; Lane6: A431 cell lysate; Lane7: Jurkat cell lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗抗增殖细胞核抗原(PCNA)单克隆抗体 Western Blot; Sample: Lane1: Mouse Testis lysate; Lane2: Mouse Small intestine lysate; Lane3: Rat Spleen lysate; Lane4: Porcine Heart lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Spleen lysate; Lane7: Bovine Cerebrum lysate; Lane8: Bovine Heart lysate; Lane9: Canine Liver lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗抗增殖细胞核抗原(PCNA)单克隆抗体 Western Blot; Sample: Lane1: Cavia Heart lysate; Lane2: Cavia Liver lysate; Lane3: Cavia Spleen lysate; Lane4: Caprine Cerebrum lysate; Lane5: Caprine Heart lysate; Lane6: Caprine Liver lysate; Lane7: Gallus Heart lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对PCNA的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别PCNA。

用法

Western blotting: 0.0003-0.1ug/ml;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠), Canis familiaris; Canine (Dog,犬) 应用(仅供研究使用,不用于临床诊断!)
RPA591Mi01 抗增殖细胞核抗原(PCNA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA591Mi01 抗增殖细胞核抗原(PCNA)多克隆抗体 WB; IHC; ICC; IP.
MAA591Mi22 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
MAA591Mi23 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
CAA591Mi22 抗抗增殖细胞核抗原(PCNA)单克隆抗体 Loading Control of WB
MAA591Mi24 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
MAA591Mi21 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
SEA591Mi 抗增殖细胞核抗原(PCNA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA591Mi 抗增殖细胞核抗原(PCNA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Tissue and Cell Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects [Pubmed:27233913]
Food and Chemical Toxicology Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088]
Life Sciences Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096]
Archives of Physiology and Biochemistry Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216]
European journal of medicinal chemistry Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504]
Antioxidants (Basel). The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets [Pubmed: 31426309]
Antioxidants Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian … [Pubmed: 31600920]
TOXICOLOGY Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124]
BIOMEDICINE & PHARMACOTHERAPY Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939]
Sci Rep Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916]
J Cell Sci Local DNA synthesis is critical for DNA repair during oocyte maturation [34415018]
Stem Cell Rev Rep Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic … [34859376]
Naunyn-Schmiedeberg's Archives Inflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus … [Pubmed:35715546]
Antioxidants Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … [Pubmed:35204140]
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … []
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … []
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