抗微管蛋白β(TUBb)单克隆抗体
Anti-Tubulin Beta (TUBb) Monoclonal Antibody
TUB-B; TUBB5; TUBB1; M40
- 编号CAB870Mi22
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度0.65mg/mL
- 且适物种Hamster (Chinese hamster，仓鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，猪), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，绵羊), Chicken (Gallus，鸡)
- 应用Loading Control of WB
- 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 716 ¥ 1672 ¥ 2388 ¥ 5970 ¥ 23880
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human MCF7 cell;
Primary Ab: 40µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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Figure. Western Blot Sample: Recombinant TUBb
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Western Blot analysis of TUBb in various cell lines
Lane1: HL60 whole cell lysate
Lane2: THP-1 whole cell lysate
Lane3: MDA-MB-435S whole cell lysate
Lane4: T-47D whole cell lysate
Lysates/proteins at 20µg per lane.
Primary Ab: anti-TUBb antibody (CAB870Mi22) at 0.3ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique.
Calculated MW: 50KD
Observed MW: 50KD
Exposure time: 1 min -
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Canine Kidney lysate; Lane4: Gallus Cerebrum lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Cerebrum lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Cavia Kidney lysate; Lane10: Caprine Kidney lysate
Primary Ab: 1µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: DU145 cell lysate; Lane2: HL60 cell lysate; Lane3: THP1 cell lysate; Lane4: OS-RC-2 cell lysate; Lane5: HEC-1B cell lysate; Lane6: MDA-MB-435S cell lysate; Lane7: T-47D cell lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Heart lysate; Lane3: Mouse Cerebellum lysate; Lane4: Porcine Cerebrum lysate; Lane5: Bovine Kidney lysate; Lane6: Rabbit Pancreas lysate
Primary Ab: 0.0003µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对TUBb的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别TUBb。
用法
Western blotting: 0.0003-0.1ug/ml
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CAB870Mi01 | 抗微管蛋白β(TUBb)多克隆抗体 | Loading Control of WB. |
| CAB870Mi22 | 抗微管蛋白β(TUBb)单克隆抗体 | Loading Control of WB |
| SEB870Mi | 微管蛋白β(TUBb)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB870Mi | 微管蛋白β(TUBb)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| archives of pharmacal research | Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues [pubmed:27747473] |
| archiv der pharmazie (Weinheim). | New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. [pubmed:28150327] |
| ACS Applied Materials & Interfaces | Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture [pubmed:28112513] |
| Future Medicinal Chemistry | Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents [Pubmed:29779400] |
| Future Medicinal Chemistry | Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization [Pubmed:29966433] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167] |
| Journal of Enzyme Inhibition and Medicinal Chemistry | Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors [Pubmed: 30191744] |
| European Journal of Medicinal Chemistry | Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study [Pubmed: 31536891] |
| ACS Sensors | Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform [Pubmed: 32207606] |
| Bioorganic Chemistry | Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their … [Pubmed: 32325332] |
| BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats [Pubmed: 33028437] |
| European Journal of Medicinal Chemistry | Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon … [Pubmed: 33158579] |
| J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells [33300466] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
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