丙酮酸脱氢酶α(PDHA1)多克隆抗体
Polyclonal Antibody to Pyruvate dehydrogenase alpha 1 (PDHA1)
PDHCE1A; PHE1A; PHE1-A; PDHa; PDH-A; Pyruvate Dehydrogenase(lipoamide)Alpha 1; Pyruvate Dehydrogenase E1 Component Subunit Alpha,Aomatic Form,Mitochondrial; Pyruvate Dehydrogenase Alpha
- 编号PAA776Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA776Hu01-丙酮酸脱氢酶α(PDHA1)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/ml
- 且适物种Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，猪)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human PDHa Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human PDHA1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20ug/ml Rabbit Anti-Human PDHA1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human PDHA1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant PDHa, Human.
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Western Blot; Sample: Lane1: Human Liver lysate; Lane2: Human Hela cell lysate; Lane3: Human ECV304 cell lysate;
Primary Ab: 2µg/mL Rabbit Anti-Human PDHa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PDHa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PDHa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PDHa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human ECV304 cell lysate; <br/>Primary Ab: 1µg/ml Rabbit Anti-Human PDHa Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PDHA1 Polyclonal Antibody (Catalog PAA776Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对PDHA1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别PDHA1。
用法
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
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相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA776Hu01 | 丙酮酸脱氢酶α(PDHA1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA776Hu01 | 丙酮酸脱氢酶α(PDHA1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA776Hu22 | 丙酮酸脱氢酶α(PDHA1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA776Hu | 丙酮酸脱氢酶α(PDHA1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA776Hu | 丙酮酸脱氢酶α(PDHA1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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