8-羟基脱氧鸟苷(8-OHdG)检测试剂盒(化学发光免疫分析法)

CLIA Kit for 8-Hydroxydeoxyguanosine (8-OHdG)

8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG

  • 8-羟基脱氧鸟苷(8-OHdG)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 8-羟基脱氧鸟苷(8-OHdG)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 8-羟基脱氧鸟苷(8-OHdG)检测试剂盒(化学发光免疫分析法) 实验结果图
  • CCA660Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测8-羟基脱氧鸟苷(8-OHdG),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的8-羟基脱氧鸟苷(8-OHdG)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 87-101 91
EDTA plasma(n=5) 89-103 96
heparin plasma(n=5) 93-103 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的8-羟基脱氧鸟苷(8-OHdG),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中8-羟基脱氧鸟苷(8-OHdG)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 86-95% 79-97% 85-101%
EDTA plasma(n=5) 88-95% 79-94% 81-99% 80-96%
heparin plasma(n=5) 98-105% 87-98% 87-99% 79-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将8-羟基脱氧鸟苷(8-OHdG)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的8-羟基脱氧鸟苷(8-OHdG)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CEA660Ge 8-羟基脱氧鸟苷(8-OHdG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA660Ge 8-羟基脱氧鸟苷(8-OHdG)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA660Ge 8-羟基脱氧鸟苷(8-OHdG)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Yonsei Med J. Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. [Pubmed: 24142649]
J Histochem Cytochem Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats [PubMed: 26385569]
Diabetes/Metabolism Research and Reviews Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of … [doi:10.1002]
Nutrition Journal Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study [Pubmed:27071648]
Diabetology & Metabolic Syndrome Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection [pmc:PMC4855822]
UVaDOC Dieta hipercalórica e hipoxia intermitente crónica como modelos de obesidad y apnea obstructiva del sueño. Significado de la leptina en el control respiratorio [Tesis1149-161108.pdf]
Journal of Diabetes Mellitus The Activity of Serum 8-Iso-Prostaglandin F2α as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia [profile:Wesam_Nasef3]
Indian Journal of Hematology and Blood Transfusion Oleuropein Ameliorates Cisplatin-induced Hematological Damages Via Restraining Oxidative Stress and DNA Injury [article:10.1007]
Journal of Diabetes Research Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-TemporalStudy: Oxidative Stress and Diabetic Salivary Glands. [pubmed:27478848]
Front Physiol. Oxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats. [pubmed:28184199]
Archives of Oral Biology Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats [pubmed:28926744]
BioMed Research International Oxidative modification of biomolecules in the non-stimulated and stimulated saliva of patients with morbid obesity treated with bariatric surgery [10.1155:2017]
Oncotarget Chemopreventive effects of atractylenolide II on mammary tumorigenesis via activating Nrf2-ARE pathway [pubmed:29100404]
International Journal of Molecular Sciences Antioxidant Defence, Oxidative Stress and Oxidative Damage in Saliva, Plasma and Erythrocytes of Dementia Patients. Can Salivary AGE be a Marker of Dementia? [pubmed:29053628]
Antioxidants and Redox Signaling Chemokine Receptor CXCR4 Plays a Crucial Role in Mediating Oxidative StressInduced Podocyte Injury [10.1089:ars.2016.6758]
Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats [ISSN:1940-5901/IJCEM0048822]
Biological Trace Element Research Protective Effect of Origanum Oil on Alterations of Some Trace Elements and Antioxidant Levels Induced by Mercuric Chloride in Male Rats [pubmed:28616770]
HAYATI Journal of Biosciences Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult SpragueeDawley Rats [10.1016/j.hjb.2017.08.007]
Scientific Reports Risk of glomerular filtration rate decline in patients with hypertrophic cardiomyopathy and obstructive sleep apnoea [pubmed:29234143]
Journal of Pain Research Sex differences in complex regional pain syndrome type I (CRPS-I) in mice [pubmed:28831269]
HAYATI Journal of Biosciences Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague–Dawley Rats [10.1016/j.hjb.2017.08.007]
Oxidative Medicine and Cellular Longevity Effect of N-Acetylcysteine on Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in a Rat Model of Insulin Resistance [Pubmed:29636851]
Oxidative medicine and cellular longevity Exposure to Ti4Al4V Titanium Alloy Leads to Redox Abnormalities, Oxidative Stress, and Oxidative Damage in Patients Treated for Mandible Fractures [Pubmed:30013720]
Journal of Cardiovascular Development and Disease Oxidative DNA Damage and Carotid Intima Media Thickness as Predictors of Cardiovascular Disease in Prediabetic Subjects [Pubmed:29518984]
Reproductive Biology Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring [Pubmed:30005909]
Oxidative medicine and cellular longevity Redox Balance, Antioxidant Defense, and Oxidative Damage in the Hypothalamus and Cerebral Cortex of Rats with High Fat Diet-Induced Insulin Resistance []
Journal of Pharmacological Reports Journal of Pharmacological Reports [Pubmed: 27489887]
Diabetes & Metabolic Syndrome: Clinical Research & Reviews Cross-sectional correlates of oxidative stress and inflammation with glucose intolerance in prediabetes []
BioMed Research International Oral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet … []
Oxidative Medicine and Cellular Longevity Luteolin Attenuates Cardiac Ischemia/Reperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function []
Oxidative Medicine and Cellular Longevity Dendrobium Officinale Polysaccharides Protect against MNNG-Induced PLGC in Rats via Activating the NRF2 and Antioxidant Enzymes HO-1 and NQO-1 []
International Journal of Pharmaceutical Research & Allied Sciences Prophylactic Impact of Beta vulgaris L in Ameliorating Cyclosporine A-Induced Hepatotoxicity in Rats. []
Journal of Diabetes and Metabolism Assessment of 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a Diagnostic Marker for Diabetic Nephropathy in Type 1 Egyptian Diabetic Patients []
Journal of Food Biochemistry Dietary supplementation of barley and/or dates attenuate hypercholesterolemic‐induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic … [Pubmed: 31373029]
environmental science and pollution research Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains [Pubmed: 31468353]
Oxidative Medicine and Cellular Longevity Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population [Pubmed: 32215181]
Journal of Clinical Medicine Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents [Pubmed: 32079369]
Frontiers in Pharmacology Hydrogen Sulfide Attenuates Particulate Matter-Induced Emphysema and Airway Inflammation Through Nrf2-Dependent Manner [Pubmed: 32116706]
Odontology Salivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitis [Pubmed: 32065311]
FREE RADICAL RESEARCH Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide … [Pubmed: 32985285]
JOURNAL OF FOOD SCIENCE Lycopene protects neuroblastoma cells against oxidative damage via depression of ER stress [Pubmed: 32885410]
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
Lipids Health Dis Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism … [Pubmed: 32487177]
Theranostics Rescuing Dicer expression in inflamed colon tissues alleviates colitis and prevents colitis-associated tumorigenesis [Pubmed: 32483416]
Journal of Clinical Medicine Systemic Redox Imbalance in Patients with Chronic Granulomatous Disease [Pubmed: 32397350]
Oxidative Medicine and Cellular Longevity NAC Supplementation of Hyperglycemic Rats Prevents the Development of Insulin Resistance and Improves Antioxidant Status but Only Alleviates General … [Pubmed: 33274008]
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross … [Pubmed: 32509152]
oxidative medine cellular longevity Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β … [Pubmed: 32617139]
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS Induction of genotoxicity and mutagenic potential of heavy metals in Thai occupational workers [Pubmed: 32928371]
Journal of Functional Foods Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets []
Journal of Diabetes Research Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China []
The potential protective role of gamma-irradiated vaccine versus Punica granatum treatment against murine trichinellosis []
Mutat Res Gen Tox En []
Electroacupuncture ameliorates motor dysfunction via inhibiting p66Shc-mediated oxidative stress and endoplasmic reticulum stress in rats with spinal cord … []
Journal of Skin and Stem Cell 8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin Treatment []
Redox Biol Biomarkers of nucleic acid oxidation¨Ca summary state-of-the-art [33579665]
Theranostics Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage [33897894]
Exp Ther Med Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats [34093766]
chemosphere Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective [33662726]
Food Funct. Sestrin2 is involved in Nrf2-regulated antioxidative signaling pathway in luteolin-induced prevention of the diabetic rat heart from ischemia/reperfusion injury [33900303]
Aquaculture Reports Growth performance, antioxidant capacity, tissue fatty acid composition and lipid metabolism of juvenile green mud crab Scylla paramamosain in response to?¡­ []
Ann Behav Med Role of Yoga and Its Plausible Mechanism in the Mitigation of DNA Damage in Type-2 Diabetes: A Randomized Clinical Trial [34180500]
Cancer Manag Res Association of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal Cancer [34408493]
The Journal of Clinical Endocrinology & Metabolism High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα [34562085]
Ecotoxicol Environ Saf Chromium induced neurotoxicity by altering metabolism in zebrafish larvae [34781135]
Toxins Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy [34437430]
J Int Med Res Body mass index is associated with Helicobacter pylori infection and increased oxidative DNA damage in an obese population [Pubmed:35209724]
Ann Transplant Biochemical and Molecular Studies of Different Parameters as Markers for Nephropathy in Type 1 Diabetic Patients [Pubmed:34751188]
Oxidative Medicine and Cellular Longevity Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway [Pubmed:35602095]
留言咨询