促肾上腺皮质激素(ACTH)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Adrenocorticotropic Hormone (ACTH)

Corticotropin; Adrenocorticotropin

  • 促肾上腺皮质激素(ACTH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 促肾上腺皮质激素(ACTH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 促肾上腺皮质激素(ACTH)检测试剂盒(化学发光免疫分析法) 实验结果图
  • CCA836Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测促肾上腺皮质激素(ACTH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的促肾上腺皮质激素(ACTH)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-91 80
EDTA plasma(n=5) 80-102 99
heparin plasma(n=5) 78-101 82

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的促肾上腺皮质激素(ACTH),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中促肾上腺皮质激素(ACTH)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 93-105% 81-97% 86-98%
EDTA plasma(n=5) 82-91% 78-103% 86-94% 89-98%
heparin plasma(n=5) 86-105% 89-98% 95-102% 91-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将促肾上腺皮质激素(ACTH)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的促肾上腺皮质激素(ACTH)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
CEA836Mu 促肾上腺皮质激素(ACTH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA836Mu 促肾上腺皮质激素(ACTH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA836Mu 促肾上腺皮质激素(ACTH)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA836Mu11 促肾上腺皮质激素(ACTH)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA836Mu11 促肾上腺皮质激素(ACTH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Respir Res. Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. [PubMed: 20403205]
Toxicology Letters Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345]
Mol Med Rep. Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. [Pubmed:24682498]
Peptides Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411]
Journal of Animal and Feed Sciences Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages [Researchgate:Source]
International Journal of Immunopathology & Pharmacology Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638]
Cell Tissue Res CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release [PubMed: 26572542]
Neuroscience Letters Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866]
Neural Regeneration Research Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. [Pubmed:27212922]
Environmental Science and Pollution Research Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway [Pubmed:27102619]
Sci Rep. Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland [Pubmed:27109116]
Sci Rep. High activity of the stress promoter contributes to susceptibility to stress in the tree shrew [Pubmed:27125313]
brain research bulletin Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. [pubmed:28161195]
中国临床药理学与治疗学 肉蔻五味丸抗抑郁作用及机制研究 [9787]
Neuroscience Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093]
Toxicology Mixture effects of azole fungicides on the adrenal gland in a broad dose range [pubmed:28454766]
Biomedicine & Pharmacotherapy Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway [pubmed:28892788]
Hormones and Behavior Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium … [Pubmed:30025718]
Frontiers in Pharmacology Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … [Pubmed:30038568]
Molecular and Cellular Endocrinology Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells [10.1016:j.mce.2018.07.007]
Frontiers in Behavioral Neuroscience Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress [Pubmed:30079015]
Placenta Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue [Pubmed:29941176]
BMC Veterinary Research Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … [Pubmed:29540178]
European Journal of Applied Physiology Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia [Pubmed: 30564880]
Cyber Leninka ТЭС-ТЕРАПИЯ КАК МЕТОД ПРЕДУПРЕЖДЕНИЯ ДЕЗАДАПТАЦИИ У САМЦОВ КРЫС С ВЫСОКОЙ СТРЕССОУСТОЙЧИВОСТЬЮ []
Serbian Journal of Experimental and Clinical Research Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance []
Scientific Reports Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition [Pubmed: 31015500]
alcoholism-clinical and experimental research Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure [Pubmed: 31329297]
Hormones and Behavior Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression [Pubmed: 32165183]
Prog Neuropsychopharmacol Biol Psychiatry Progress in Neuropsychopharmacology & Biological Psychiatry [Pubmed: 17134810]
Evid Based Complement Alternat Med So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo [Pubmed: 33224257]
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory [Pubmed: 32682873]
Aging Cell Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582]
neurochemical research Anxiolytic and Anti‑depressive Like Effects of Translocator Protein (18 kDa) Ligand YL‑IPA08 in a Rat Model of Postpartum Depression [Pubmed: 32383026]
Behavioral Ecology and Sociobiology  Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax … []
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­ [33389158]
International Journal of?Nanomedicine Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­ [33762821]
J Am Heart Assoc Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest [33960200]
Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients [33936350]
留言咨询