B-细胞淋巴瘤因子2(Bcl2)检测试剂盒(化学发光免疫分析法)

CLIA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)

B-Cell CLL/Lymphoma 2

  • B-细胞淋巴瘤因子2(Bcl2)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • B-细胞淋巴瘤因子2(Bcl2)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • B-细胞淋巴瘤因子2(Bcl2)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA778Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测B-细胞淋巴瘤因子2(Bcl2),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的B-细胞淋巴瘤因子2(Bcl2)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-102 93
EDTA plasma(n=5) 78-94 83
heparin plasma(n=5) 84-91 88

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的B-细胞淋巴瘤因子2(Bcl2),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中B-细胞淋巴瘤因子2(Bcl2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-101% 78-91% 99-105% 78-95%
EDTA plasma(n=5) 82-94% 81-101% 92-101% 88-96%
heparin plasma(n=5) 88-95% 90-103% 81-95% 80-91%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将B-细胞淋巴瘤因子2(Bcl2)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的B-细胞淋巴瘤因子2(Bcl2)与连接于固相载体上的抗体结合,然后加入生物素化的B-细胞淋巴瘤因子2(Bcl2)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的B-细胞淋巴瘤因子2(Bcl2)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA778Hu01 B-细胞淋巴瘤因子2(Bcl2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA778Hu01 B-细胞淋巴瘤因子2(Bcl2)多克隆抗体 WB,IHC,ICC/IF
LAA778Hu81 B-细胞淋巴瘤因子2(Bcl2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA778Hu71 B-细胞淋巴瘤因子2(Bcl2)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA778Hu22 B-细胞淋巴瘤因子2(Bcl2)单克隆抗体 WB; IHC; ICC; IP.
MAA778Hu21 B-细胞淋巴瘤因子2(Bcl2)单克隆抗体 WB; IHC; ICC; IP.
MAA778Hu23 B-细胞淋巴瘤因子2(Bcl2)单克隆抗体 WB; IHC; ICC; IP.
MAA778Hu24 B-细胞淋巴瘤因子2(Bcl2)单克隆抗体 WB; IHC; ICC; IP.
MAA778Hu25 B-细胞淋巴瘤因子2(Bcl2)单克隆抗体 WB; IHC; ICC; IP.
SEA778Hu B-细胞淋巴瘤因子2(Bcl2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA778Hu B-细胞淋巴瘤因子2(Bcl2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA778Hu B-细胞淋巴瘤因子2(Bcl2)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Anticancer Research Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233]
International Journal of Health Sciences Neurotoxic effect of lead on rats: Relationship to Apoptosis [NCBI: PMC3883608]
Interactive cardiovascular and thoracic surgery Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosin [Pubmed: 24327573]
J Physiol Biochem. Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats [Pubmed:24752458]
Chem Biol Interact.? Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activities [Pubmed:25289771]
Pediatric Cardiology Serum Bcl-2 Values in Children with Pulmonary Hypertension [Pubmed:25381623]
Histochem Cell Biol. Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis [Pubmed:25511416]
PLOS ONE Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-γ, NF-κB and Nrf2/HO-1 Signaling Pathways [PubMed: 26196679]
Neurochem Res Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436]
European Journal of Medicinal Plants Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms [openview:626440]
IJBPAS THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS [publication:298838934]
Mol Neurobiol. Neuroprotective Effect of Nanodiamond in Alzheimer's Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling [Pubmed:26897372]
Iranian Journal of Basic Medical Sciences The ameliorative potential of ethanolic extract of propolis on hematotoxicity and structural neuronal damage in hyperthermia-exposed rats [pubmed:27746870]
Scientific Reports In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Disease [pubmed:27609215]
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease]
Journal of Ethnopharmacology Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction bydanhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms. [pubmed:27729285]
European Journal of Pharmacology Mangiferin protects against‭ intestinal ischemia/reperfusion-induced‭ liver injury:‬‬ Involvement of PPAR-‭ γ, GSK-3β and Wnt/β-catenin pathway‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬ [pubmed:28506911]
Journal of Applied Pharmaceutical Science Comparative Effects of Stevia rebaudiana and Aspartame on hepato-renal function of diabetic rats: Biochemical and Histological Approaches [59b5141c458515a5b49377cf]
Cellular Physiology and Biochemistry A Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in Rats [pubmed:28942443]
Biomedicine & Pharmacotherapy Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in rats [pubmed:28554128]
Thromb Haemost Distinctive expression signatures of serum microRNAs in ischaemic stroke and transient ischaemic attack patients [pubmed:28251236]
International Journal of Biological Macromolecules A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats [pubmed:28709897]
Molecular Neurobiology Neuroprotective Effect of Nanodiamond in Alzheimer’s Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling [pubmed:26897372]
Toxicology and Environmental Health Sciences Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin [10.1007/s13530-017-0325-1]
Biomedicine & Pharmacotherapy Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heart [pubmed:28686977]
Scientific Research Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress Disorder [paperID:82598]
Biomaterials Structural optimization and additional targets identification of antisense oligonucleotide G3139 encapsulated in a neutral cytidinyl-lipid combined with a cationic lipid … [Pubmed: 30660994]
PeerJ. Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway [Pubmed: 30723634]
博士论文 INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA []
pharmacological research Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors [Pubmed: 31048034]
Int Immunopharmacol Metallothionein-1 is associated with osteoarthritis disease activity and suppresses proinflammatory cytokines production in synovial cells [Pubmed: 31465913]
Experimental Cell Research LncRNA SNHG3 promotes clear cell renal cell carcinoma proliferation and migration by upregulating TOP2A [Pubmed: 31505165]
Toxicology LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating … [Pubmed: 32197949]
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337]
Environ Sci Pollut Res Int Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of … [Pubmed: 32915453]
Drug Chem Toxicol Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascade [Pubmed: 32869669]
Iranian Journal of phamaceutical research Potential anti-tumor activity of kefir-induced juglone and resveratrol fractions against Ehrlich ascites carcinoma-bearing BALB/c mice []
ACS APPLIED BIO MATERIALS Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy []
Folia Biologica Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats []
Current Nutrition & Food Science The Effect of Sodium Metabisulphite on Apoptosis in the Experimental Model of Parkinson's Disease []
Life Sci Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-¦Ã, NF-¦ÊB, and STAT3 crosstalk [33310046]
J Biochem Mol Toxicol Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ¡­ [33491863]
Drug Des Devel Ther Magnesium Isoglycyrrhizinate Alleviates Arsenic Trioxide-Induced Cardiotoxicity: Contribution of Nrf2 and TLR4/NF-¦ÊB Signaling Pathway [33603344]
Crystals Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent) []
BIOTECHNOLOGIES Expression of Apoptotic Markers Bcl-2 and Bax in the Vascular Wall []
Pharmacol Res Perspect [8]‐Gingerol exerts anti‐myocardial ischemic effects in rats via modulation of the MAPK signaling pathway and L‐type Ca2+ channels [34390539]
Ecotoxicol Environ Saf Lycopene attenuates zearalenone-induced oxidative damage of piglet sertoli cells through the nuclear factor erythroid-2 related factor signaling pathway [34482067]
Biomed Pharmacother Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats [34426252]
Environ Sci Pollut Res Int Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity [34729716]
Sci Rep Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026]
J Ethnopharmacol Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498]
Life Sci Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and … [Pubmed:35640776]
Life Sci Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosis [Pubmed:35636581]
Antioxidants (Basel) Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino … [Pubmed:35624893]
Experimental and Therapeutic Medicine Nephroprotective effect of magnesium isoglycyrrhizinate against arsenic trioxide‑induced acute kidney damage in mice [Pubmed:35317438]
Biointerface Research in Applied Chemistry Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats []
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