脑源性神经营养因子(BDNF)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Brain Derived Neurotrophic Factor (BDNF)

Neurotrophin; Abrineurin

  • 脑源性神经营养因子(BDNF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 脑源性神经营养因子(BDNF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 脑源性神经营养因子(BDNF)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA011Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测脑源性神经营养因子(BDNF),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的脑源性神经营养因子(BDNF)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 81-91 87
EDTA plasma(n=5) 85-101 90
heparin plasma(n=5) 92-101 96

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的脑源性神经营养因子(BDNF),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中脑源性神经营养因子(BDNF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 99-105% 93-105% 91-103%
EDTA plasma(n=5) 89-97% 83-95% 93-105% 80-97%
heparin plasma(n=5) 91-104% 86-94% 79-104% 98-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

试剂盒内容

试剂名称 数量 试剂名称 数量
96孔板(预包被) 1 96孔板覆膜 4
标准品 2 标准品稀释液 1×20mL
检测溶液A 1×120µL 检测稀释液A 1×12mL
检测溶液B 1×120µL 检测稀释液B 1×12mL
底物A 1×10mL 底物B 1×2mL
浓洗涤液 (30 × concentrate) 1×20mL 说明书 1

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

CLIA Kit for Brain Derived Neurotrophic Factor (BDNF)

实验原理

将脑源性神经营养因子(BDNF)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的脑源性神经营养因子(BDNF)与连接于固相载体上的抗体结合,然后加入生物素化的脑源性神经营养因子(BDNF)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的脑源性神经营养因子(BDNF)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA011Hu01 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA011Hu61 脑源性神经营养因子(BDNF)活性蛋白 Cell culture; Activity Assays.
EPA011Hu61 脑源性神经营养因子(BDNF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA011Hu01 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
PAA011Hu06 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
LAA011Hu71 脑源性神经营养因子(BDNF)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA011Hu81 脑源性神经营养因子(BDNF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA011Hu22 脑源性神经营养因子(BDNF)单克隆抗体 WB; IHC; ICC; IP.
SEA011Hu 脑源性神经营养因子(BDNF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA011Hu 脑源性神经营养因子(BDNF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA011Hu01 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Neurochemical Research Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. [Pubmed:26559686]
Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats [pmc:PMC4812310]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
The International Journal af Applied Research in Veterinary Medicine Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study [articles:Vol14Iss3]
J Chem Neuroanat. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. [pubmed:27914953]
Int Immunopharmacol. PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. [pubmed:27940378]
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639]
Bipolar Disorders Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … [bdi.12449]
Elsevier Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls [science:article]
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