Ⅱ型胶原(COL2)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Collagen Type II (COL2)

  • Ⅱ型胶原(COL2)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • Ⅱ型胶原(COL2)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • Ⅱ型胶原(COL2)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA572Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测Ⅱ型胶原(COL2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的Ⅱ型胶原(COL2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 86-94 91
EDTA plasma(n=5) 78-89 85
heparin plasma(n=5) 86-99 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的Ⅱ型胶原(COL2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅱ型胶原(COL2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 80-94% 84-91% 85-96%
EDTA plasma(n=5) 79-90% 79-96% 82-93% 78-95%
heparin plasma(n=5) 86-93% 98-105% 94-102% 87-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。

实验原理

将Ⅱ型胶原(COL2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Ⅱ型胶原(COL2)与连接于固相载体上的抗体结合，然后加入生物素化的Ⅱ型胶原(COL2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的Ⅱ型胶原(COL2)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
PAA572Hu01 Ⅱ型胶原(COL2)多克隆抗体 WB; IHC; ICC; IP.
SEA572Hu Ⅱ型胶原(COL2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA572Hu 抗Ⅱ型胶原抗体(Anti-COL2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA572Hu Ⅱ型胶原(COL2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

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