红细胞生成素(EPO)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Erythropoietin (EPO)

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

  • 红细胞生成素(EPO)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 红细胞生成素(EPO)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 红细胞生成素(EPO)检测试剂盒(化学发光免疫分析法) 实验结果图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测红细胞生成素(EPO),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将红细胞生成素(EPO)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的红细胞生成素(EPO)与连接于固相载体上的抗体结合,然后加入生物素化的红细胞生成素(EPO)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的红细胞生成素(EPO)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA028Hu61 红细胞生成素(EPO)活性蛋白 Cell culture; Activity Assays.
EPA028Hu61 红细胞生成素(EPO)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA028Hu01 红细胞生成素(EPO)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA028Hu01 红细胞生成素(EPO)活性蛋白 Cell culture; Activity Assays.
PAA028Hu06 红细胞生成素(EPO)多克隆抗体 WB; IHC; ICC; IP.
PAA028Hu01 红细胞生成素(EPO)多克隆抗体 WB; IHC; ICC; IP.
LAA028Hu71 红细胞生成素(EPO)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA028Hu22 红细胞生成素(EPO)单克隆抗体 WB; IHC; ICC; IP.
SEA028Hu 红细胞生成素(EPO)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA028Hu 红细胞生成素(EPO)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA028Hu01 红细胞生成素(EPO)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Endocrinology Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring [Endo: source]
Journal of Ethnopharmacology Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression [PubMed: 21333724]
PLOS ONE CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source]
The Journal of Clinical Endocrinology & Metabolism Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation [PubMed: 22948762]
Experimental & Molecular Medicine Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression [PubMed: PMC3509184]
Biochem Biophys Res Commun. Reevaluation of erythropoietin production by the nephron [Pubmed:24832733]
Nutrition. Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. [Pubmed:2528040]
J Appl Physiol (1985). Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness [Pubmed:25324511]
Expert Rev Proteomics Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone [PubMed: 26496240]
Oncotarget KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin [pubmed:26575329]
international journal of molecular sciences Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608]
Veterinary Clinical Pathology Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease [pubmed:28186645]
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease]
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