雌二醇(E2)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Estradiol (E2)

17B-Estradiol; Oestradiol; Beta-Estradiol

  • 雌二醇(E2)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 雌二醇(E2)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 雌二醇(E2)检测试剂盒(化学发光免疫分析法) 实验结果图
  • CCA461Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测雌二醇(E2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的雌二醇(E2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 85-101 92
EDTA plasma(n=5) 87-104 93
heparin plasma(n=5) 90-97 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的雌二醇(E2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中雌二醇(E2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 89-96% 91-105% 98-105% 82-98%
EDTA plasma(n=5) 82-104% 92-101% 83-97% 80-103%
heparin plasma(n=5) 85-92% 88-99% 79-105% 90-98%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分钟；
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将雌二醇(E2)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原（标准品或样本），待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的雌二醇(E2)呈负相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。

相关产品

编号 适用物种：Pan-species (General，通用) 应用(仅供研究使用，不用于临床诊断！)
CPA461Ge11 雌二醇(E2)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA461Ge21 雌二醇(E2)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA461Ge31 雌二醇(E2)钥孔血蓝蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA461Ge01 雌二醇(E2)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA461Ge71 雌二醇(E2)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA461Ge81 雌二醇(E2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA461Ge21 雌二醇(E2)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA461Ge28 雌二醇(E2)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA461Ge27 雌二醇(E2)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MEA461Ge 雌二醇(E2)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA461Ge 雌二醇(E2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA461Ge 雌二醇(E2)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA461Ge 雌二醇(E2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA461Ge 雌二醇(E2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA461Ge11 雌二醇(E2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
BioMed research international Antiosteoclastic Activity of Milk Thistle Extract after Ovariectomy to Suppress Estrogen Deficiency-Induced Osteoporosis [PubMed: PMC3678416]
Fertility and Sterility Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells [Pubmed: 23954356]
Repositório Digital Utilizacao de células-tronco adultas para tratamento de falência ovariana causada por quimioterapia em camundongas [Lume: Source]
Electromagnetic Biology and Medicine The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats [Informahealthcare: Source]
Academic Sciences Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats [Ijppsjournal: Source]
Stem Cells Int. Cell Therapy for Chemically Induced Ovarian Failure in Mice [Pubmed:Pmc4274854]
Reproductive Toxicology Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring [Pubmed:24126190]
Phytomedicine. Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in mice [Pubmed:24932975]
Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy [Turkishjournalofurology:Source]
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy [PubMed: 25996886]
Reprod Toxicol In utero [PubMed: 25530038]
oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats [PubMed: 26629940]
Environ Toxicol Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period [PubMed: 25782373]
J Endocrinol Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction [PubMed: 25770118]
Reproduction Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats [PubMed: 26556892]
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
Archives of Physiology and Biochemistry Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder [Pubmed:26934364]
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota [Pubmed:27093642]
Biology of Reproduction Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring [Pubmed:26962114]
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα [Pubmed:27413362]
Clinical and Experimental Hypertension Assessment of ADMA, estradiol, and progesterone in severe preeclampsia [Pubmed:27152507]
PLoS Genet. A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis [Pubmed:26977770]
Scientific Reports The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization [pubmed:27876896]
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373]
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389]
African Journal of Environmental Science and Technology Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania [150659]
Journal of Functional Foods Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women [S1756464617301561]
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061]
Environmental toxicology and pharmacology Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. [pubmed:28376377]
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα [pubmed:27413362]
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384]
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267]
Interact Cardiovasc Thorac Surg Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats [pubmed:29049608]
Toxicological Sciences Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666]
Reproductive Sciences Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome [pubmed:28673204]
Oncotarget Rcan2 and estradiol independently regulate body weight in female mice [pubmed:28624805]
African Journal of Environmental Science and Technology Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania [10.5897/AJEST2016.2234]
Journal of Orthopaedic Research The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice [pubmed:29058779]
Animal Production Science Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? [10.1071/ANv57n12Ab065]
Asian Journal of Biomedical and Pharmaceutical Sciences A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized Rats [ISSN 2249 – 622X]
Nanotheranostics The biocompatibility studies of polymer dots on pregnant mice and fetuses [pubmed:29071192]
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999]
Reproduction. Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring [pubmed:28687593]
Molecular Medicine Reports Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells [pubmed:28656272]
Annals of Rheumatic Diseases  FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study [Suppl_2:728.1.abstract]
Stem Cell Research & Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657]
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625]
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci]
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
Aquatic Toxicology Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations [Pubmed:29775925]
Journal of Ethnopharmacology Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model [Pubmed:29705515]
Inflammation Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female Male [Pubmed:29737476]
European Journal of Neuroscience Postnatal separation prevents the development of prenatal stress‐induced anxiety in association with changes in oestrogen receptor and oxytocin immunoreactivity in … [Pubmed:29205599]
Journal of Ovarian Research DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway [Pubmed:29321035]
Medical Science Monitor Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … [Pubmed:29674607]
Biomedicine & Pharmacotherapy Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135]
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146]
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … [Pubmed: 30606243]
journal of molecular neuroscience Possible Metabolic Alterations among Autistic Male Children: Clinical and Biochemical Approaches [Pubmed: 30600432]
Heliyon Establishment and evaluation of a novel mouse model of peri/postmenopausal depression [Doi: 10.1016/j.heliyon.2019.e01195]
Experimental and Therapeutic Medicine Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary []
Evidence-Based Complementary and Alternative Medicine iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine []
Biomedicine & Pharmacotherapy G protein-coupled estrogen receptor activation improves contractile and diastolic functions in rat renal interlobular artery to protect against renal ischemia … [Pubmed: 30784936]
Biomedicine & Pharmacotherapy Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve []
Biology of Reproduction G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … [Pubmed: 31034039]
Biomedicine & pharmacotherapy Aqueous extract of lily bulb ameliorates menopause-like behavior in ovariectomized mice with novel brain-uterus mechanisms distinct from estrogen therapy [Pubmed: 31207575]
Reproductive Biology and Endocrinology Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … [Pubmed: 31311554]
Experimental Neurology Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause [Pubmed: 31377402]
Oxidative medicine and cellular longevity Total Polysaccharides of Lily Bulb Ameliorate Menopause-Like Behavior in Ovariectomized Mice: Multiple Mechanisms Distinct from Estrogen Therapy [Pubmed: 31428228]
Journal of Ovarian Research Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718]
Cellular Signalling Effect of FSH on E2/GPR30-mediated mouse oocyte maturation in vitro [Pubmed: 31704004]
Chemosphere Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938]
Neuroscience Blocking of Estradiol Receptors ERα, ERβ and GPER During Development, Differentially Alters Energy Metabolism in Male and Female Rats [Pubmed: 31805254]
Open Access Maced J Med Sc Gambir Extract (Uncaria Gambir) Decreases Inflammatory Response and Increases Gastric Mucosal Integrity in Wistar Rats-Model Gastritis [Pubmed: 31949507]
Estradiol treatment during perinatal development alters [Pubmed: 31966342]
science of the total environment Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells [Pubmed: 32334352]
molecular nutrition & food research Lepidiline A Improves the Balance of Endogenous Sex Hormones and Increases Fecundity by Targeting HSD17B1 [Pubmed: 32239630]
Chemosphere Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … [Pubmed: 33131727]
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio) [Pubmed: 33011509]
Neuropharmacology Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084]
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH Protective effects of irbesartan and benazepril against vaginal vascular remodeling and fibrosis in female spontaneously hypertensive rats [Pubmed: 32790534]
ARCHIVES OF GYNECOLOGY AND OBSTETRICS MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043]
Antioxidants (Basel) Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model [Pubmed: 32517356]
Andrologia Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty [Pubmed: 32478927]
Neurotoxicology Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice [Pubmed: 32407858]
archives of toxicology Effect of estrogen-active compounds on the expression of RACK1 and immunological implications [Pubmed: 32328699]
Heliyon Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … [Pubmed: 33005806]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
Iranian Journal of Basic Medical Sciences Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS []
biorxiv The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice []
Moosense pedometer activity and periestrual hormone profile in relation to oestrus in crossbred cattle []
J Steroid Biochem Mol Biol Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development [33465420]
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
JOURNAL OF ETHNOPHARMACOLOGY BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose [33508368]
Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice []
Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency []
Arch Gynecol Obstet The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … [34417839]
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391]
Stem Cells Int Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … [34733331]
Eur J Pharmacol 17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice [34822791]
IBRO Neuroscience Reports Effects of Treadmill Exercise on Anxiety-like Behavior in Association with Changes in Estrogen Receptors ERα, ERβ and Oxytocin of C57BL/6J Female Mice [34746914]
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]
Nat Commun TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice [Pubmed:35177657]
Appl Biochem Biotechnol ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse Pituitary [Pubmed:35138554]
Stem Cell Research & Therapy G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and … []
Research Square Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency []
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