促卵泡素(FSH)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Follicle Stimulating Hormone (FSH)

  • 促卵泡素(FSH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 促卵泡素(FSH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 促卵泡素(FSH)检测试剂盒(化学发光免疫分析法) 实验结果图
  • CCA830Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测促卵泡素(FSH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的促卵泡素(FSH)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 87-94 91
EDTA plasma(n=5) 85-101 91
heparin plasma(n=5) 93-101 97

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的促卵泡素(FSH),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中促卵泡素(FSH)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 82-96% 92-101% 85-101%
EDTA plasma(n=5) 83-101% 78-104% 83-97% 79-94%
heparin plasma(n=5) 83-91% 80-102% 82-104% 97-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将促卵泡素(FSH)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的促卵泡素(FSH)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA830Mu01 促卵泡素(FSH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA830Mu01 促卵泡素(FSH)多克隆抗体 WB; IHC; ICC; IP.
LAA830Mu71 促卵泡素(FSH)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA830Mu 促卵泡素(FSH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA830Mu 促卵泡素(FSH)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA830Mu 促卵泡素(FSH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA830Mu 促卵泡素(FSH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA830Mu 促卵泡素(FSH)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
International Journal of Clinical and Experimental Medicine Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene [Ijcem: IJCEM1102004]
Science Alert Reproductive Toxicity of Tobacco Shisha Smoking on Semen Parameters and Hormones Levels among Adult Egyptian Men [SciAlert: source]
EXCLI Journal Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats [Excli: Source]
International Journal of Molecular and Cellular Medicine Central effects of camphor on GnRH and sexual hormones in male rat [Pubmed: 24551777]
Urologia internationalis Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats [Pubmed: 24051982]
Electromagnetic Biology and Medicine The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats [Informahealthcare: Source]
Academic Sciences Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats [Ijppsjournal: Source]
Mol Cell Endocrinol. Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats [Pubmed:24316378]
Endocrinology FoxO1 Is a Negative Regulator of FSHβ Gene Expression in Basal and GnRH-Stimulated Conditions in Female [Pubmed:24437485]
Andrologia Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis [Pubmed:24576220]
Environmental Toxicology and Pharmacology Endocrine disrupting effects of dichlorodiphenyltrichloroethane analogues on gonadotropin hormones in pituitary gonadotrope cells [Pubmed:24814263]
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141]
Genet Mol Biol. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility [Pubmed:25505846]
Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy [Turkishjournalofurology:Source]
oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats [PubMed: 26629940]
Andrologia Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis [PubMed: 24576220]
European Journal of Pharmacology The effect of R-(-)-deprenyl administration on reproductive parameters of rat males [PubMed: 25725114]
Andrologia Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats [PubMed: 26123866]
Reprod Toxicol Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats [PubMed: 26476359]
The Journal of The Society for Reproduction and Fertility Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure [Pubmed:27026714]
Biology of Reproduction Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring [Pubmed:26962114]
Andrologia Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. [Pubmed:26123866]
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373]
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389]
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061]
Andrologia Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. [pubmed:28000943]
Biology of Reproduction Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. [pubmed:26962114]
J Endocrinol. Impact of uteroplacental insufficiency on postnatal rat male gonad. [pubmed:27885054]
Int J Pharm. New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. [pubmed:28232269]
Environmental Science & Technology Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis [pubmed:28731686]
Journal of Medicinal Chemistry Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity [pubmed:28958147]
Society of Toxicology Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells [pubmed:29149324]
Reproductive Toxicology Vitamin D deficiency impairs testicular development and spermatogenesis in mice [pubmed:28655646]
The Journal of Biological Chemistry. Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. [pubmed:28578315]
The Journal of Toxicological Sciences High sensitivity of testicular function to titanium nanoparticles [pubmed:28496042]
Journal of Orthopaedic Research The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice [pubmed:29058779]
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999]
Reproduction. Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring [pubmed:28687593]
Annals of Rheumatic Diseases  FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study [Suppl_2:728.1.abstract]
Stem Cell Research & Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657]
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625]
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
Biology of Reproduction Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss [Pubmed:29351580]
Journal of Ovarian Research DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway [Pubmed:29321035]
Medical Science Monitor Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … [Pubmed:29627845]
Human & Experimental Toxicology Palladium nanoparticle effects on endocrine reproductive system of female rats [Pubmed:29405765]
A Pex13 knockout in germ cells induces a spermatogenic arrest [volltexte:2018/8921/]
Biomedicine & Pharmacotherapy Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135]
Stem Cells Translational Medicine Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats [Pubmed: 30569668]
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146]
Experimental and Therapeutic Medicine Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary []
Biomedicine & Pharmacotherapy Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve []
Biology of Reproduction G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … [Pubmed: 31034039]
International Journal of Reproductive Biomedicine The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study. []
Reproductive Biology and Endocrinology Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … [Pubmed: 31311554]
Andrologia Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats [Pubmed: 31353623]
Journal of Ovarian Research Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718]
Genesis Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterus [Pubmed: 31815354]
Chemosphere Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938]
Medicina Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology [Pubmed: 32120970]
Chemosphere Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … [Pubmed: 33131727]
BIOLOGICAL RESEARCH Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance … [Pubmed: 33109277]
ARCHIVES OF GYNECOLOGY AND OBSTETRICS MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043]
Mol Med Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic … [Pubmed: 32698821]
Antioxidants (Basel) Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model [Pubmed: 32517356]
chemosphere N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats [Pubmed: 32447106]
Neurotoxicology Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice [Pubmed: 32407858]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
ACS Applied Bio Materials Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice []
Iranian Journal of Basic Medical Sciences Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS []
Medical Journal of Cairo University Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management []
biorxiv The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice []
Drug-Herb Interaction Between Selective Estrogen Receptor Modulators and Estrogenic Herbal Medicine Er-Xian Decoction in Bone in Vivo and in Vitro []
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
advanced biomedical research The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome [33457340]
Endocrinology Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment [33507237]
JOURNAL OF ETHNOPHARMACOLOGY BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose [33508368]
Journal of Ethnopharmacology Water extract of Er-xian decoction selectively exerts estrogenic activities and interacts with SERMs in estrogen-sensitive tissues [33823166]
Toxicol Appl Pharmacol Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion [33965371]
researchsquare Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells []
Scientific reports Regulation of Uterine Function During Estrous Cycle, Anestrus Phase and Pregnancy by Steroids in Red Deer (Cervus elaphus L.) []
Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice []
Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency []
JOURNAL OF INHERITED METABOLIC DISEASE Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in … [34494673]
Bioengineered MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1 [34637688]
Free Radical Biology and Medicine Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion [34610361]
Prostate Endothelial dysfunction after androgen deprivation therapy and the possible underlying mechanisms [34570375]
Microbiome Polyamine metabolism links gut microbiota and testicular dysfunction [34758869]
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391]
Stem Cells Int Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … [34733331]
Cell Death Dis METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice [34689175]
Theriogenology Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with … [Pubmed:35063921]
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]
Nat Commun TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice [Pubmed:35177657]
Sci Total Environ Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression [Pubmed:35276145]
Ecotoxicology and Environmental Safety Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero [Pubmed:35679725]
Stem Cell Research & Therapy G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and … []
Research Square Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency []
BMC Veterinary Research A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats []
留言咨询