凝溶胶蛋白(GSN)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Gelsolin (GSN)

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

  • 凝溶胶蛋白(GSN)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 凝溶胶蛋白(GSN)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 凝溶胶蛋白(GSN)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA372Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测凝溶胶蛋白(GSN),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的凝溶胶蛋白(GSN)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 82-96 85
EDTA plasma(n=5) 84-92 89
heparin plasma(n=5) 84-102 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的凝溶胶蛋白(GSN),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中凝溶胶蛋白(GSN)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 78-89% 78-99% 80-91%
EDTA plasma(n=5) 84-99% 98-105% 98-105% 97-104%
heparin plasma(n=5) 98-105% 84-98% 84-103% 82-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将凝溶胶蛋白(GSN)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的凝溶胶蛋白(GSN)与连接于固相载体上的抗体结合,然后加入生物素化的凝溶胶蛋白(GSN)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的凝溶胶蛋白(GSN)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA372Hu02 凝溶胶蛋白(GSN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu05 凝溶胶蛋白(GSN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu06 凝溶胶蛋白(GSN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu03 凝溶胶蛋白(GSN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu04 凝溶胶蛋白(GSN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu01 凝溶胶蛋白(GSN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA372Hu61 凝溶胶蛋白(GSN)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA372Hu21 凝溶胶蛋白(GSN)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA372Hu05 凝溶胶蛋白(GSN)多克隆抗体 WB; IHC; ICC; IP.
PAA372Hu04 凝溶胶蛋白(GSN)多克隆抗体 WB; IHC; ICC; IP.
PAA372Hu03 凝溶胶蛋白(GSN)多克隆抗体 WB; IHC; ICC; IP.
PAA372Hu02 凝溶胶蛋白(GSN)多克隆抗体 WB; IHC; ICC; IP.
PAA372Hu01 凝溶胶蛋白(GSN)多克隆抗体 WB; IHC; ICC; IP.
PAA372Hu08 凝溶胶蛋白(GSN)多克隆抗体 WB; IHC; ICC; IP.
LAA372Hu71 凝溶胶蛋白(GSN)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA372Hu27 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC; ICC; IP.
MAA372Hu29 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC; ICC; IP.
MAA372Hu22 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC; ICC; IP.
MAA372Hu24 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC
MAA372Hu21 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC; ICC; IP.
MAA372Hu23 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC; ICC; IP.
MAA372Hu25 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC; ICC; IP.
MAA372Hu26 凝溶胶蛋白(GSN)单克隆抗体 WB; IHC; ICC; IP.
SEA372Hu 凝溶胶蛋白(GSN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA372Hu 凝溶胶蛋白(GSN)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA372Hu 凝溶胶蛋白(GSN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA372Hu 凝溶胶蛋白(GSN)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA372Hu01 凝溶胶蛋白(GSN)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA372Hu01 凝溶胶蛋白(GSN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Neurochmical Research Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy [SpringerLink: 3585r8h46q7r0171]
Journal of Neuroinflammation Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury [BioMed: 17422094]
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563]
European Journal of Obstetrics & Gynecology and Reproductive Biology Investigation of the actin scavenging system in pre-eclampsia [Pubmed: 24239294]
The Annals of Thoracic Surgery Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases [Pubmed: 24565402]
BMC Anesthesiol.? The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study [Pubmed:Pmc4132929]
Cell Biochemistry and Biophysics Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury [Pubmed:25164111]
Neurosci Lett Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease [PubMed: 25864780]
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509]
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509]
Medicina Intensiva Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. [pubmed:28365030]
Journal of Alzheimers Disease Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720]
World Journal of Pediatrics Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children [Pubmed:29427164]
Biomedicines Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various … [Pubmed:35327525]
留言咨询