生长激素(GH)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Growth Hormone (GH)

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

  • 生长激素(GH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 生长激素(GH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 生长激素(GH)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA044Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测生长激素(GH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的生长激素(GH)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 79-96 82
EDTA plasma(n=5) 89-101 95
heparin plasma(n=5) 87-104 92

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的生长激素(GH),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中生长激素(GH)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 83-104% 81-98% 91-101%
EDTA plasma(n=5) 95-102% 85-92% 78-101% 86-101%
heparin plasma(n=5) 94-103% 78-99% 83-93% 85-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将生长激素(GH)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的生长激素(GH)与连接于固相载体上的抗体结合,然后加入生物素化的生长激素(GH)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的生长激素(GH)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA044Ra01 生长激素(GH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA044Ra01 生长激素(GH)多克隆抗体 WB; IHC; ICC; IP.
MAA044Ra21 生长激素(GH)单克隆抗体 WB; IHC; ICC; IP.
SEA044Ra 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA044Ra 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA044Ra 生长激素(GH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA044Ra01 生长激素(GH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Neuroscience Letters The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats [PubMed: 19765635]
Cellular Reprogramming Changes in the Somatotrophic Axis in Genetically Identical Dogs [PubMed: 21740269]
Clinical Cancer Research The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status [PubMed: 21642381]
General and Comparative Endocrinology Growth hormone expression and neuroprotective activity in a quail neural retina cell line [ScienceDirect: S0016648009002457]
Nutrition and Metabolism Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis [Nutrition&Metabolism: source]
Veterinary Recprd Case Report Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism [Bmj: Source]
Journal of the American Animal Hospital Association GH Deficiency in a Dalmatian Puppy with Megaesophagus [Pubmed: 24051259]
Biotechnology and Bioprocess Engineering Functional expression of bovine growth hormone gene in Pleurotus eryngii [Springer: Source]
Buletin Veteriner Udayana Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali [Unud:Source]
Veterinary World Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows [Veterinaryworld:Source]
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831]
Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks [Pubmed:26948666]
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87]
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87]
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
Jurnal Riset Akuakultur TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA [1329]
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