生长激素(GH)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Growth Hormone (GH)

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

  • 生长激素(GH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 生长激素(GH)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 生长激素(GH)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA044Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测生长激素(GH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的生长激素(GH)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 85-99 94
EDTA plasma(n=5) 96-104 101
heparin plasma(n=5) 90-103 97

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的生长激素(GH),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中生长激素(GH)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 78-103% 93-101% 84-96%
EDTA plasma(n=5) 93-103% 88-102% 79-104% 95-103%
heparin plasma(n=5) 81-104% 78-89% 95-105% 96-103%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将生长激素(GH)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的生长激素(GH)与连接于固相载体上的抗体结合,然后加入生物素化的生长激素(GH)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的生长激素(GH)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA044Hu01 生长激素(GH)活性蛋白 Cell culture; Activity Assays.
EPA044Hu61 生长激素(GH)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA044Hu01 生长激素(GH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA044Hu06 生长激素(GH)多克隆抗体 WB; IHC; ICC; IP.
PAA044Hu01 生长激素(GH)多克隆抗体 WB; IHC; ICC; IP.
MAA044Hu22 生长激素(GH)单克隆抗体 WB; IHC; ICC; IP.
LAA044Hu72 生长激素(GH)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA044Hu 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA044Hu 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA044Hu 抗生长激素抗体(Anti-GH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA044Hu 生长激素(GH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA044Hu01 生长激素(GH)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA044Hu01 生长激素(GH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Neuroscience Letters The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats [PubMed: 19765635]
Cellular Reprogramming Changes in the Somatotrophic Axis in Genetically Identical Dogs [PubMed: 21740269]
Clinical Cancer Research The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status [PubMed: 21642381]
General and Comparative Endocrinology Growth hormone expression and neuroprotective activity in a quail neural retina cell line [ScienceDirect: S0016648009002457]
Nutrition and Metabolism Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis [Nutrition&Metabolism: source]
Veterinary Recprd Case Report Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism [Bmj: Source]
Journal of the American Animal Hospital Association GH Deficiency in a Dalmatian Puppy with Megaesophagus [Pubmed: 24051259]
Biotechnology and Bioprocess Engineering Functional expression of bovine growth hormone gene in Pleurotus eryngii [Springer: Source]
Buletin Veteriner Udayana Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali [Unud:Source]
Veterinary World Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows [Veterinaryworld:Source]
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831]
Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks [Pubmed:26948666]
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87]
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87]
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
Jurnal Riset Akuakultur TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA [1329]
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