肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Hepatocyte Growth Factor (HGF)

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

  • 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) 实验结果图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测肝细胞生长因子(HGF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的肝细胞生长因子(HGF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 85-94 90
EDTA plasma(n=5) 91-99 95
heparin plasma(n=5) 85-97 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的肝细胞生长因子(HGF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中肝细胞生长因子(HGF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 78-93% 86-94% 79-96%
EDTA plasma(n=5) 93-102% 79-95% 97-105% 82-96%
heparin plasma(n=5) 78-88% 81-90% 86-97% 84-93%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。

实验原理

将肝细胞生长因子(HGF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的肝细胞生长因子(HGF)与连接于固相载体上的抗体结合，然后加入生物素化的肝细胞生长因子(HGF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的肝细胞生长因子(HGF)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。

相关产品

编号 适用物种：Bos taurus; Bovine (Cattle，牛) 应用(仅供研究使用，不用于临床诊断！)
RPA047Bo01 肝细胞生长因子(HGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA047Bo01 肝细胞生长因子(HGF)多克隆抗体 WB; IHC; ICC; IP.
MAA047Bo21 肝细胞生长因子(HGF)单克隆抗体 WB; IHC; ICC; IP.
SEA047Bo 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA047Bo 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA047Bo 肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

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