乳脂球表皮生长因子8(MFGE8)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)
HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8
- 编号SCB286Hu
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围31.2-2,000pg/mL
- 灵敏度最小可检测剂量小于等于12.2pg/mL.
- 样本类型Serum, plasma, cell lysates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3352 ¥ 4788 ¥ 21546 ¥ 40698 ¥ 335160
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特异性
本试剂盒用于检测乳脂球表皮生长因子8(MFGE8),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的乳脂球表皮生长因子8(MFGE8)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 93-102 | 97 |
EDTA plasma(n=5) | 85-102 | 97 |
heparin plasma(n=5) | 91-101 | 96 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的乳脂球表皮生长因子8(MFGE8),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中乳脂球表皮生长因子8(MFGE8)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-97% | 92-99% | 95-105% | 87-102% |
EDTA plasma(n=5) | 98-105% | 92-99% | 95-102% | 87-101% |
heparin plasma(n=5) | 81-96% | 83-101% | 94-103% | 98-105% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。
实验原理
将乳脂球表皮生长因子8(MFGE8)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的乳脂球表皮生长因子8(MFGE8)与连接于固相载体上的抗体结合,然后加入生物素化的乳脂球表皮生长因子8(MFGE8)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的乳脂球表皮生长因子8(MFGE8)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB286Hu01 | 乳脂球表皮生长因子8(MFGE8)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB286Hu01 | 乳脂球表皮生长因子8(MFGE8)多克隆抗体 | WB; IHC; ICC; IP. |
MAB286Hu22 | 乳脂球表皮生长因子8(MFGE8)单克隆抗体 | WB; IHC; ICC; IP. |
SEB286Hu | 乳脂球表皮生长因子8(MFGE8)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB286Hu | 乳脂球表皮生长因子8(MFGE8)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB286Hu | 乳脂球表皮生长因子8(MFGE8)等多因子检测试剂盒(流式荧光发光法) | FLIA Assay for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Fertility and Sterility | Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells. [Pubmed: 24262600] |
U.S. Patent Application | Biomarker for amyloid-b-related neurological disorders [US20130302838] |
IUBMB Life. | Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status. [pubmed:28067023] |
Journal of diabetes investigation | Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus. [pubmed:28035763] |
The Journal of Steroid Biochemistry and Molecular Biology | Modifications of Western-type diet regarding protein, fat and sucrose levels as modulators of steroid metabolism and activity in liver [pubmed:27471150] |
Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling [pubmed:28263910] |
Oncotarget | Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response. [pubmed:28489606] |
Molecular and Cellular Neuroscience | TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway [10.1016/j.mcn.2017.08.006] |
Amyloid | Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein [Pubmed: 31615282] |
clinical and translational medicine | Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis [33634976] |