降钙素原(PCT)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Procalcitonin (PCT)

Pro-Calcitonin

  • 降钙素原(PCT)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 降钙素原(PCT)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 降钙素原(PCT)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA689Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测降钙素原(PCT),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的降钙素原(PCT)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 81-95 90
EDTA plasma(n=5) 90-104 94
heparin plasma(n=5) 80-93 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的降钙素原(PCT),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中降钙素原(PCT)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 94-102% 80-95% 95-105%
EDTA plasma(n=5) 91-98% 99-105% 79-102% 86-101%
heparin plasma(n=5) 97-105% 78-90% 92-105% 79-92%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将降钙素原(PCT)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的降钙素原(PCT)与连接于固相载体上的抗体结合,然后加入生物素化的降钙素原(PCT)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的降钙素原(PCT)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA689Hu02 降钙素原(PCT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA689Hu01 降钙素原(PCT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA689Hu03 降钙素原(PCT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA689Hu21 降钙素原(PCT)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA689Hu01 降钙素原(PCT)多克隆抗体 WB; IHC; ICC; IP.
PAA689Hu02 降钙素原(PCT)多克隆抗体 WB; IHC; ICC; IP.
PAA689Hu08 降钙素原(PCT)多克隆抗体 WB; IHC; ICC; IP.
LAA689Hu71 降钙素原(PCT)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA689Hu28 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
MAA689Hu21 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
MAA689Hu29 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
MAA689Hu22 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
MAA689Hu23 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
MAA689Hu27 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
MAA689Hu24 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
MAA689Hu25 降钙素原(PCT)单克隆抗体 WB; IHC; ICC; IP.
SEA689Hu 降钙素原(PCT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA689Hu 降钙素原(PCT)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA689Hu 降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA689Hu01 降钙素原(PCT)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA689Hu01 降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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Egyptian Journal of Bronchology Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children [Essbronchology: Source]
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Egyptian Journal of Medical Microbiology Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis [Ejmm:Source]
Nigerian Medical Journal Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis [Pubmed:25657488]
Wiley online library The use and value of procalcitonin in solid organ transplantation [PubMed: 25996831]
Balkan Med J Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia [PubMed: 26185718]
The Journal of Clinical Endocrinology & Metabolism Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay [PubMed: 26647152]
Journal of Human Hypertension Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension [PubMed: 26040439]
Medical Science Monitor Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection. [Pubmed:26772168]
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Cellular Physiology and Biochemistry Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure [Pubmed:29913443]
Archives of Medical Science Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase [Pubmed:29593801]
Journal of Thoracic Disease Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung [Pubmed:29707313]
Asian Journal of Psychiatry Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients [Pubmed:29723719]
European Review for Medical and Pharmacological Sciences The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341]
Medicina Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection [Pubmed: 33080909]
MEDIATORS OF INFLAMMATION Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase [Pubmed: 32410851]
Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes []
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Bone Res Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment [Pubmed:35087025]
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