肾素(REN)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Renin (REN)

angiotensinogenase

  • 肾素(REN)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 肾素(REN)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 肾素(REN)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA889Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测肾素(REN),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的肾素(REN)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-99 95
EDTA plasma(n=5) 88-104 97
heparin plasma(n=5) 79-96 91

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的肾素(REN),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中肾素(REN)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 78-94% 82-105% 95-104%
EDTA plasma(n=5) 88-95% 94-105% 97-104% 79-93%
heparin plasma(n=5) 80-99% 97-105% 91-101% 84-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将肾素(REN)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的肾素(REN)与连接于固相载体上的抗体结合,然后加入生物素化的肾素(REN)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的肾素(REN)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA889Hu01 肾素(REN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA889Hu01 肾素(REN)多克隆抗体 WB; IHC; ICC; IP.
MAA889Hu22 肾素(REN)单克隆抗体 WB; IHC; ICC; IP.
SEA889Hu 肾素(REN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA889Hu 肾素(REN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Hypertension Prenatal overexposure to glucocorticoids programs renal 11β-hydroxysteroid dehydrogenase type 2 expression and salt-sensitive hypertension in the rat [PubMed: 21045727]
The Journal of Physiology Recombinant human erythropoietin in humans down-regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate [Physoc: 194241]
The Journal of Physiology Erythropoietin down‐regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate in humans [PubMed: 20724370]
Pathobiology Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer Cells [Karger: 000330169]
Journal of Cardiovascular Pharmacology The Angiotensin Receptor Blocker, Azilsartan Medoxomil (TAK-491), Suppresses Vascular Wall Expression of Plasminogen Activator Inhibitor Type-I Protein Potentially Facilitating the Stabilization of Atherosclerotic Plaques [LWW: 08000]
Kidney International Multiple and large simple renal cysts are associated with prehypertension and hypertension. [Pubmed: 23389415]
Human Physiology Estimation of the parameters of the renin-angiotensin-aldosterone system, water-electrolyte metabolism, and endothelial function in newborns of women with chronic hypertension [Springer:Source]
Journal of Pharmacological Sciences Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats [Pubmed:24366191]
Liver International VSL#3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride-induced cirrhosis [Pubmed:24750552]
Gynecological Endocrinology Immunological and hemostasiological disorders in women with ovarian hyperstimulation syndrome [Pubmed:25200828]
Oxidative Medicine and Cellular Longevity Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice [Pubmed:27200147]
PLoS One Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria [Pubmed:27166674]
PLOS ONE Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. [pubmed:27391973]
Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054]
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