组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)

EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor

  • 组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA552Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测组织金属蛋白酶抑制因子1(TIMP1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的组织金属蛋白酶抑制因子1(TIMP1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-101 93
EDTA plasma(n=5) 86-101 97
heparin plasma(n=5) 89-96 92

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的组织金属蛋白酶抑制因子1(TIMP1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中组织金属蛋白酶抑制因子1(TIMP1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 81-88% 92-102% 87-94% 80-90%
EDTA plasma(n=5) 99-105% 96-104% 78-92% 83-93%
heparin plasma(n=5) 83-95% 97-104% 92-99% 84-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将组织金属蛋白酶抑制因子1(TIMP1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的组织金属蛋白酶抑制因子1(TIMP1)与连接于固相载体上的抗体结合,然后加入生物素化的组织金属蛋白酶抑制因子1(TIMP1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的组织金属蛋白酶抑制因子1(TIMP1)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA552Mu02 组织金属蛋白酶抑制因子1(TIMP1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA552Mu01 组织金属蛋白酶抑制因子1(TIMP1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA552Mu01 组织金属蛋白酶抑制因子1(TIMP1)多克隆抗体 WB; IHC; ICC; IP.
PAA552Mu02 组织金属蛋白酶抑制因子1(TIMP1)多克隆抗体 WB; IHC; ICC; IP.
LAA552Mu71 组织金属蛋白酶抑制因子1(TIMP1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA552Mu 组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA552Mu 组织金属蛋白酶抑制因子1(TIMP1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

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