25-羟基维生素D3(HVD3)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for 25-Hydroxyvitamin D3 (HVD3)

25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D

  • 25-羟基维生素D3(HVD3)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 25-羟基维生素D3(HVD3)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对25-羟基维生素D3(HVD3)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Pan-species (General，通用) 应用(仅供研究使用，不用于临床诊断！)
CPA915Ge11 25-羟基维生素D3(HVD3)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA915Ge21 25-羟基维生素D3(HVD3)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA915Ge01 25-羟基维生素D3(HVD3)多克隆抗体 WB; IHC; ICC; IP.
LAA915Ge71 25-羟基维生素D3(HVD3)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA915Ge21 25-羟基维生素D3(HVD3)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA915Ge 25-羟基维生素D3(HVD3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA915Ge 25-羟基维生素D3(HVD3)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA915Ge11 25-羟基维生素D3(HVD3)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Allergy & Clinical Immunology Decreased serum LL-37 and vitamin D3 levels in atopic dermatitis: relationship between IL-31 and oncostatin M [Wiley: source]
Tianjin Med J 成人哮喘血清维生素D 水平与肺功能相关因素分析 [Jwk_Tjyy: Cn]
Journal of Functional Foods Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women [S1756464617301561]
J Nutr Biochem. Chronic maternal calcium and 25-hydroxyvitamin D deficiency in Wistar rats programs abnormal hepatic gene expression leading to hepatic steatosis in female offspring. [pubmed:28219837]
 in vivo Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level [pubmed:28064234]
Asia pacific allergy Serum 25-hydroxyvitamin D inversely associated with blood eosinophils in patients with persistent allergic rhinitis. [pubmed:29094019]
Biomed Research International  Effects of Vitamin D Restricted Diet Administered during Perinatal and Postnatal Periods on the Penis of Wistar Rats [Pubmed:29850540]
Clin Implant Dent Relat Res. Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseases [Pubmed: 31407857]
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in … [Pubmed: 33065276]
The Journal of Obstetrics and Gynecology of India The Effects of Follicular Fluid 25 (OH) D Concentration on Intrafollicular Estradiol Level, Oocyte Quality, and Fertilization Rate in Women Who Underwent IVF Program []
留言咨询