血小板反应蛋白解整合素金属肽酶4(ADAMTS4)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

ADMP-1; Aggrecanase-1

  • 血小板反应蛋白解整合素金属肽酶4(ADAMTS4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 血小板反应蛋白解整合素金属肽酶4(ADAMTS4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对血小板反应蛋白解整合素金属肽酶4(ADAMTS4)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

参考文献

杂志 参考文献
Biomedicine & Pharmacotherapy ADAMTS4 level in patients with stable coronary artery disease and acute coronary syndromes [ScienceDirect: S0753332209002017]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Anadolu Kardiyol Derg. A novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes. [Pubmed:25592103]
Osteoarthritis and Cartilage Lack of anti-inflammatory and anti-catabolic effects on basal inflamed osteoarthritic chondrocytes or synoviocytes by adipose stem cell-conditioned medium [PubMed: 26521751]
Osteoarthritis and Cartilage Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs [Pubmed:26802547]
Journal of Cellular and Molecular Medicine VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts [Pubmed:26818776]
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748]
The American journal of pathology Healthy and Osteoarthritic Synovial Fibroblasts Produce a Disintegrin and Metalloproteinase with Thrombospondin Motifs 4, 5, 7, and 12: Induction by IL-1β and Fibronectin and Contribution to Cartilage Damage. [pubmed:27449198]
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748]
American Journal of Translational Research Diagnostic utility of fluorogenic peptide-conjugated Au nanoparticle probe corroborated by rabbit model of mild cartilage injury and panel of osteoarthritic … [Pubmed: 30210670]
Journal of Clinical Medicine The Effects of TNF-α Inhibition on the Metabolism of Cartilage: Relationship between KS, HA, HAPLN1 and ADAMTS4, ADAMTS5, TOS and TGF-β1 Plasma … [Pubmed:35407621]
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