爱帕琳肽(APLN)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Apelin (APLN)

XNPEP2; APEL; APJ endogenous ligand

  • 爱帕琳肽(APLN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 爱帕琳肽(APLN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对爱帕琳肽(APLN)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

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参考文献

杂志 参考文献
Clin Exp Hypertens. Individual and Concomitant Effects of Cardioprotective Programs on Cardiac Apelinergic System and Oxidative State in └-NAME-Induced Hypertension [Informahealthcare: Source]
African Journal of Pharmacy and Pharmacology Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. [Academicjournals: Source]
Zahedan Journal of research in Medical Sciences Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Hypertension Rats [Zjrms: Source]
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658]
Mol Metab TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice [PubMed: 26500845]
Journal of medicinal food Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. [pubmed:28170279]
Arch Physiol Biochem Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696]
preprints Serum Apelin and Galactin-3 in Preeclampsia in Iraq [Pubmed: 32538210]
Journal of Diabetes Research The Potential Role of Chemerin, Lipocalin 2, and Apelin in the Diagnosis and Pathophysiology of Gestational Diabetes Mellitus []
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