110 KSB022Ge11 | β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法) | Pan-species (General,通用) USCN(武汉优尔生商贸有限公司)

β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Beta-Hydroxybutyric Acid (bHB)

OHB; BHBA; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid; β-Hydroxybutyric acid

  • β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对β-羟丁酸(bHB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPB022Ge11 β-羟丁酸(bHB)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPB022Ge21 β-羟丁酸(bHB)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAB022Ge01 β-羟丁酸(bHB)多克隆抗体 WB; IHC; ICC; IP.
CEB022Ge β-羟丁酸(bHB)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB022Ge β-羟丁酸(bHB)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB022Ge11 β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Endocrinology Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression [Endocrine:Source]
Chem Commun (Camb) A bioelectronic system for insulin release triggered by ketone body mimicking diabetic ketoacidosis in vitro [PubMed: 25846235]
PLoS One Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine [pubmed:27501393]
Buffalo Bulletin Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes []
Journal of Integrative Agriculture Temporal dynamics of nutrient balance, plasma biochemical and immune traits, and liver function in transition dairy cows []
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