脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)

Neurotrophin; Abrineurin

  • 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对脑源性神经营养因子(BDNF)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
RPA011Po01 脑源性神经营养因子(BDNF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA011Po01 脑源性神经营养因子(BDNF)多克隆抗体 WB; IHC; ICC; IP.
MAA011Po26 脑源性神经营养因子(BDNF)单克隆抗体 WB; IHC; ICC; IP.
MAA011Po21 脑源性神经营养因子(BDNF)单克隆抗体 WB; IHC; ICC; IP.
LAA011Po76 脑源性神经营养因子(BDNF)单克隆抗体(生物素标记) WB; IHC; ICC.
KSA011Po01 脑源性神经营养因子(BDNF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Toxicological Sciences Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains [PubMed: 21436126]
Journal of the Neurological Sciences Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord [ScienceDirect: S0022510X12001219]
International Conference on Bioscience Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage [Psrcentre: 212059]
Neuroscience Letters Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice [ScienceDirect: S0304394010015788]
BioMed Research International Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. [NCBI: PMC3925622]
Behavioural Brain Research The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor [Pubmed:25446810]
Neurochemical Research Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. [Pubmed:26559686]
Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats [pmc:PMC4812310]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
The International Journal af Applied Research in Veterinary Medicine Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study [articles:Vol14Iss3]
J Chem Neuroanat. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. [pubmed:27914953]
Int Immunopharmacol. PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. [pubmed:27940378]
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639]
Bipolar Disorders Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … [bdi.12449]
Elsevier Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls [science:article]
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