肌肽(Car)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Carnosine (Car)

β-Alanyl-L-Histidine

  • 肌肽(Car)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 肌肽(Car)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对肌肽(Car)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Springerplus Dietary supplemental vitamin B6 increases carnosine and anserine concentrations in the heart of rats [PubMed: 26101732]
Mol Nutr Food Res Zinc supplementation influences genomic stability biomarkers, antioxidant activity, and zinc transporter genes in an elderly Australian population with low zinc status [PubMed: 25755079]
Pediatric Diabetes The effect of 12 weeks carnosine supplementation on renal functional integrity and oxidative stress in pediatric patients with diabetic nephropathy: a randomized placebo-controlled trial [pubmed:28744992]
Arch. Anim. Breed The effect of genotype and muscle type on the physico-chemical characteristics and taurine, carnosine and L-carnitine concentration in lamb meat [33473367]
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