非神经元性烯醇化酶(ENO1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Enolase 1 (ENO1)

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

  • 非神经元性烯醇化酶(ENO1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 非神经元性烯醇化酶(ENO1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对非神经元性烯醇化酶(NNE)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB449Mu01 非神经元性烯醇化酶(ENO1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB449Mu01 非神经元性烯醇化酶(ENO1)多克隆抗体 WB,IHC,ICC/IF
LAB449Mu71 非神经元性烯醇化酶(ENO1)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB449Mu81 非神经元性烯醇化酶(ENO1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEB449Mu 非神经元性烯醇化酶(ENO1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB449Mu 非神经元性烯醇化酶(ENO1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB449Mu01 非神经元性烯醇化酶(ENO1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Analytical Chemistry Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor [PubMed: 20557064]
Translational Research Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385]
Journal of Proteomics Putative salivary biomarkers useful to differentiate patients with fibromyalgia [Pubmed:29654921]
BioMed Research International The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis [Pubmed: 33062681]
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
Clin Proteomics Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma [Pubmed:35610567]
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