成纤维细胞生长因子15(FGF15)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Fibroblast Growth Factor 15 (FGF15)

  • 成纤维细胞生长因子15(FGF15)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 成纤维细胞生长因子15(FGF15)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对成纤维细胞生长因子15(FGF15)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APL154Ra01 成纤维细胞生长因子15(FGF15)活性蛋白 Cell culture; Activity Assays.
RPL154Ra01 成纤维细胞生长因子15(FGF15)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAL154Ra01 成纤维细胞生长因子15(FGF15)多克隆抗体 WB; IHC; ICC; IP.
MAL154Ra22 成纤维细胞生长因子15(FGF15)单克隆抗体 WB; IHC; ICC; IP.
MAL154Ra23 成纤维细胞生长因子15(FGF15)单克隆抗体 WB; IHC; ICC; IP.
MAL154Ra24 成纤维细胞生长因子15(FGF15)单克隆抗体 WB; IHC; ICC; IP.
MAL154Ra25 成纤维细胞生长因子15(FGF15)单克隆抗体 WB; IHC; ICC; IP.
MAL154Ra27 成纤维细胞生长因子15(FGF15)单克隆抗体 WB; IHC; ICC; IP.
MAL154Ra21 成纤维细胞生长因子15(FGF15)单克隆抗体 WB; IHC; ICC; IP.
WEL154Ra 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEL154Ra 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEL154Ra 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LML154Ra 成纤维细胞生长因子15(FGF15)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSL154Ra01 成纤维细胞生长因子15(FGF15)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Endocrinology. Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21 [PubMed: 25774553]
Inflammatory Bowel Diseases Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation [pubmed:27580383]
J Lipid Res. Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis. [pubmed:28130274]
BMC Endocrine Disorders The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance [10.1186/s12902-017-0211-5]
Journal of Physiology and Biochemistry The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in mice [Pubmed:29468415]
Developmental Cell FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis [Pubmed: 30745141]
Endocrinology Interactions between the gravitostat and the fibroblast growth factor system for the regulation of body weight []
Food & Function Lactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axis [Pubmed: 31626262]
Journal of Translational Medicine Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation [Pubmed: 33036625]
Chin Med Si-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis [34736501]
Int J Biol Sci Dynamics of the gut-liver axis in rats with varying fibrosis severity [Pubmed:35637968]
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