碱性成纤维细胞生长因子(FGF2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2)

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

  • 碱性成纤维细胞生长因子(FGF2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 碱性成纤维细胞生长因子(FGF2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对碱性成纤维细胞生长因子(FGF2)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA551Hu01 碱性成纤维细胞生长因子(FGF2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA551Hu01 碱性成纤维细胞生长因子(FGF2)活性蛋白 Cell culture; Activity Assays.
CPA551Hu21 碱性成纤维细胞生长因子(FGF2)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA551Hu01 碱性成纤维细胞生长因子(FGF2)多克隆抗体 WB; IHC; ICC; IP.
LAA551Hu71 碱性成纤维细胞生长因子(FGF2)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA551Hu81 碱性成纤维细胞生长因子(FGF2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA551Hu22 碱性成纤维细胞生长因子(FGF2)单克隆抗体 WB; IHC; ICC; IP.
SEA551Hu 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA551Hu 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA551Hu11 碱性成纤维细胞生长因子(FGF2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Molecular Pharmacology Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats [Pubmed: 22091745]
Science Alert Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source]
Nanoscale Research Letters Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. [PubMed: 22827927]
PLoS One Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis [PubMed: PMC3771850]
International Journal of Molecular Sciences Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104]
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Implant Dentistry Effects of Hemostatic Agents on Fibroblast Cells [Lww:Source]
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Tumor Biology Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
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Biomaterials Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars [Pubmed:26774564]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
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Medical Science Monitor 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [898221]
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