葡萄糖转运蛋白4(GLUT4)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Glucose Transporter 4 (GLUT4)

SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive

  • 葡萄糖转运蛋白4(GLUT4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 葡萄糖转运蛋白4(GLUT4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对葡萄糖转运蛋白4(GLUT4)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPC023Ra01 葡萄糖转运蛋白4(GLUT4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC023Ra01 葡萄糖转运蛋白4(GLUT4)多克隆抗体 WB; IHC; ICC; IP.
MAC023Ra21 葡萄糖转运蛋白4(GLUT4)单克隆抗体 WB; IHC; ICC; IP.
SEC023Ra 葡萄糖转运蛋白4(GLUT4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC023Ra 葡萄糖转运蛋白4(GLUT4)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSC023Ra01 葡萄糖转运蛋白4(GLUT4)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Polar Record Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development [Cambridge: Source]
The International Journal of Biochemistry & Cell Biology Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogs [Pubmed:25236492]
Psychoneuroendocrinology Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats [PubMed: 26143539]
PLOS ONE Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug [PubMed: 26262991]
American Journal of Animal and Veterinary Sciences Metabolic Features of Heart Failure with Different Etiology [ofsp11075]
Journal of Pharmaceutical Negative Results Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation [Doi: 10.4103/jpnr.JPNR_14_18]
eureka herba indonesia Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue []
eureka herba indonesia The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study []
eureka herba indonesia Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan []
Eureka Herba Indonesia Potential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against?¡­ []
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