95 KSA565Hu01 | 硫酸肝素蛋白聚糖(HSPG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Homo sapiens (Human,人) USCN(武汉优尔生商贸有限公司)

硫酸肝素蛋白聚糖(HSPG)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG)

SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan

  • 硫酸肝素蛋白聚糖(HSPG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 硫酸肝素蛋白聚糖(HSPG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对硫酸肝素蛋白聚糖(HSPG)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA565Hu01 硫酸肝素蛋白聚糖(HSPG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA565Hu01 硫酸肝素蛋白聚糖(HSPG)多克隆抗体 WB; IHC; ICC; IP.
LAA565Hu71 硫酸肝素蛋白聚糖(HSPG)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA565Hu81 硫酸肝素蛋白聚糖(HSPG)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA565Hu22 硫酸肝素蛋白聚糖(HSPG)单克隆抗体 WB; IHC; ICC; IP.
SEA565Hu 硫酸肝素蛋白聚糖(HSPG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA565Hu 硫酸肝素蛋白聚糖(HSPG)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA565Hu01 硫酸肝素蛋白聚糖(HSPG)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Journal of Applied Pharmaceutical Science Cytotoxic effects of antiglypican-3 against HepG3 cell line [Ebscohost: Source]
Journal of Reproductive Immunology Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patients [Pubmed: 24661950]
European journal of pharmacology Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme [Pubmed: 24530413]
European Journal of Pharmacology Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma [Pubmed:25449037]
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257]
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669]
Apmis Circulating syndecans during critical illness. [pubmed:28256016]
Biomedicine & Pharmacotherapy Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and [Pubmed:29669302]
Drug Delivery Glioma targeting peptide modified apoferritin nanocage [Pubmed:29726297]
Anaesthesia Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934]
Animals Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period … []
LIFE SCIENCES Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients [33781826]
Genet Mol Biol A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate [34042151]
Poultry Science Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡­ [33518110]
J Gerontol A Biol Sci Med Sci The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … [34423832]
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