硝基酪氨酸(NT)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Nitrotyrosine (NT)

3-Nitro-L-Tyrosine; 3-Nitrotyrosine

  • 硝基酪氨酸(NT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 硝基酪氨酸(NT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 硝基酪氨酸(NT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品图片
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对硝基酪氨酸(NT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Molecular Medicine Reports 3-Nitrotyrosine, a biomarker for cardiomyocyte apoptosis induced by diabetic cardiomyopathy in a rat model [Pubmed: 23970331]
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
J Histochem Cytochem Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats [PubMed: 26385569]
canadian journal of physiology and pharmacology Protective effects of Telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuro-inflammation and amyloidogenesis: possible role of brain derived neurotrophic factor [10.1139:cjpp-2017-0042]
Orphanet journal of rare diseases The cardiovascular phenotype of adult patients with phenylketonuria [Pubmed: 31492166]
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990]
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