胃蛋白酶(PP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Pepsin (PP)

  • 胃蛋白酶(PP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 胃蛋白酶(PP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对胃蛋白酶(PP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
CPA632Hu21 胃蛋白酶(PP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
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PAA632Hu09 胃蛋白酶(PP)多克隆抗体 WB; IHC; ICC; IP.
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IEA632Hu 胃蛋白酶(PP)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSA632Hu11 胃蛋白酶(PP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Radiology and Oncology LEVEL OF PEPSIN AND BILE ACIDS IN THE SALIVA OF PATIENTS WITH GLOTTIS T1 CARCINOMA [Szd:Source]
Radiology and Oncology Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer [Pubmed:Pmc4362607]
Clin Otolaryngol Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux – a prospective comparative study [PubMed: 25516364]
journal of pediatric gastroenterology and nutrition Does Positioning Affect Tracheal Aspiration of Gastric Content in Ventilated Infants? [PubMed: 25313850]
Journal of Voice Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakia [pubmed:28756936]
Sensors (Basel) Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed Electrode [Pubmed:29882917]
The Tohoku Journal of Experimental Medicine Association between Pepsin in Bronchoalveolar Lavage Fluid and Prognosis of Chronic Fibrosing Interstitial Lung Disease []
Respiratory Research Pulmonary aspiration in preschool children with cystic fibrosis [Pubmed: 30558606]
Tanaffos Association of Bile Acid and Pepsin Micro-aspiration with Chronic Obstructive Pulmonary Disease Exacerbation [Pubmed: 31423141]
Sensors Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux [Pubmed: 31935973]
BIOSCIENCE REPORTS Presence of pepsin in laryngeal tissue and saliva in benign and malignant neoplasms [Pubmed: 33103719]
OTOLARYNGOLOGY-HEAD AND NECK SURGERY Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord Leukoplakia [Pubmed: 32692278]
American Journal of Otolaryngology Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps [Pubmed: 32889371]
The relationship between laryngopharyngeal reflux based on pepsin value and clinical characteristics of laryngeal cancer patients []
Med Sci Monit The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and 24-Hour?¡­ [33220027]
Annals of Esophagus Pepsin properties, structure, and its accurate measurement: a narrative review []
Laryngoscope The Impact of Laryngopharyngeal Reflux on Occurrence and Clinical Course of Recurrent Respiratory Papillomatosis [34338331]
Pulmonology Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumonia [Pubmed:35715334]
Neuromodulation: Technology at the Neural Interface Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome [Pubmed:35088760]
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