S100钙结合蛋白A12(S100A12)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for S100 Calcium Binding Protein A12 (S100A12)

CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6

  • S100钙结合蛋白A12(S100A12)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A12(S100A12)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对S100钙结合蛋白A12(S100A12)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Oryctolagus cuniculus (Rabbit,兔) 应用(仅供研究使用,不用于临床诊断!)
RPB080Rb01 S100钙结合蛋白A12(S100A12)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB080Rb51 S100钙结合蛋白A12(S100A12)多克隆抗体 WB; IHC; ICC; IP.
PAB080Rb01 S100钙结合蛋白A12(S100A12)多克隆抗体 WB; IHC; ICC; IP.
SEB080Rb S100钙结合蛋白A12(S100A12)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB080Rb S100钙结合蛋白A12(S100A12)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSB080Rb01 S100钙结合蛋白A12(S100A12)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
Inflammation Research Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes [Pubmed: 24378957]
Dig Dis Sci The Circulating Level of Soluble Receptor for Advanced Glycation End Products Displays Different Patterns in Ulcerative Colitis and Crohn’s Disease: A Cross-Sectional Study [PubMed: 25757448]
The Journal of Clinical Endocrinology & Metabolism Low Endogenous Secretory Receptor for Advanced Glycation End-Products Levels Are Associated With Inflammation and Carotid Atherosclerosis in Prediabetes [Pubmed:26885882]
Nutrition, Metabolism, and Cardiovascular Diseases High intake of dietary advanced glycation end-products is associated with increased arterial stiffness and inflammation in subjects with type 2 diabetes. [pubmed:28958695]
The Journal of Clinical Endocrinology & Metabolism HbA1c Identifies Subjects With Prediabetes and Subclinical Left Ventricular Diastolic Dysfunction. [pubmed:28973588]
Journal of Immunology Research A Granulocyte-Specific Protein S100A12 as a Potential Prognostic Factor Affecting Aggressiveness of Therapy in Patients with Juvenile Idiopathic Arthritis [Doi: 10.1155/2018/5349837]
Al-Azhar Assiut Medical Journal The role of serum S100A12 (calgranulin C) as a diagnostic marker in Egyptian patients with irritable bowel syndrome and ulcerative colitis [Doi: 10.4103/AZMJ.AZMJ_52_18]
Acta Diabetologica Analysis of S100A12 plasma levels in hyperlipidemic subjects with or without familial hypercholesterolemia [Pubmed: 30963307]
Cells 1 h Postload Glycemia Is Associated with Low Endogenous Secretory Receptor for Advanced Glycation End Product Levels and Early Markers of Cardiovascular … []
Biomolecules . Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study [Pubmed: 32549327]
Mediators of Inflammation Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris []
Animals (Basel) The Serum and Saliva Proteome of Dogs with Diabetes Mellitus [33271797]
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