95 KSE912Mu01 | 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Mus musculus (Mouse,小鼠) USCN(武汉优尔生商贸有限公司)

沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Sirtuin 1 (SIRT1)

SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

  • 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对沉默调节蛋白1(SIRT1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPE912Mu01 沉默调节蛋白1(SIRT1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPE912Mu02 沉默调节蛋白1(SIRT1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAE912Mu01 沉默调节蛋白1(SIRT1)多克隆抗体 WB; IHC; ICC; IP.
PAE912Mu02 沉默调节蛋白1(SIRT1)多克隆抗体 WB; IHC; ICC; IP.
LAE912Mu71 沉默调节蛋白1(SIRT1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEE912Mu 沉默调节蛋白1(SIRT1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LME912Mu 沉默调节蛋白1(SIRT1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSE912Mu01 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
PLoS One. SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases [Plos: Source]
PLOS ONE A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats [Pubmed:25501750]
Journal of Alzheimer's Disease Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment [Pubmed:25589731]
Respirology Increases in peripheral SIRT1: A new biological characteristic of asthma [PubMed: 26040995]
PLOS ONE A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: [PubMed: 25785999]
Endocrine Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease. [pubmed:27522360]
Naunyn Schmiedebergs Arch Pharmacol The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury [pubmed:28361247]
 International journal of cardiology A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease [pubmed:28043667]
International Journal of Cardiology Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial [pubmed:28029409]
Hepatology International Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population [pubmed:29189974]
Toxicology and Applied Pharmacology Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454]
Chemico-biological Interactions Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction [pubmed:28919305]
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … [Pubmed:30037068]
Experimental Gerontology Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia [Pubmed:30071285]
Kidney and Blood Pressure Research The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713]
Journal of International Medical Research Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study [Pubmed: 30556451]
BioMed Research International Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic … []
学位论文 Influência do tratamento da doença periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doença arterial coronária []
Journal of Physics D: Applied Physics Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility []
Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517]
Biomed Research International Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of … [Pubmed: 30956976]
Journal of Diabetes and its Complications Vasohibin-1 in T2DM with different UACR []
Clinical Interventions in Aging The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis [Pubmed: 32214805]
Compr Psychiatry Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor [Pubmed: 33045669]
Сибирский федеральный университет Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте []
Journal of Cereal Science Dietary buckwheat enhances sirtuin1 without calorie restriction []
Clin Interv Aging Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study [33447019]
Antioxidants The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡­ [33396247]
Int Urol Nephrol Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease [33864594]
Neurochem Int Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ¡­ [34052296]
Toxicol Appl Pharmacol Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories [34174262]
Food Chem Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats [34763933]
Life Sci Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease [34774622]
Int J Mol Sci Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats [Pubmed:35163435]
J Clin Med Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter? [Pubmed:35683374]
Cell & Bioscience OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress []
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