多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对多配体蛋白聚糖1(SDC1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB966Ra02 多配体蛋白聚糖1(SDC1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB966Ra01 多配体蛋白聚糖1(SDC1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB966Ra01 多配体蛋白聚糖1(SDC1)多克隆抗体 WB; IHC; ICC; IP.
LAB966Ra81 多配体蛋白聚糖1(SDC1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB966Ra21 多配体蛋白聚糖1(SDC1)单克隆抗体 WB; IHC; ICC; IP.
SEB966Ra 多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB966Ra01 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Molecular and Cellular Biochemistry Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction [SpringerLink: a201w3l18k257462]
Inflammation Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats [Springer: Source]
Atherosclerosis. Damage of the endothelial glycocalyx in chronic kidney disease [Pubmed:24727235]
Journal of Trauma and Acute Care Surgery Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury [Pubmed:25058256]
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257]
Mediators Inflamm Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding [PubMed: 26199464]
Transplantation Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. [PubMed: 26371596]
Clinical and Experimental Nephrology Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury [pubmed:27405618]
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669]
Danish National RESEARCH DATABASE Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats [10.1089]
留言咨询