聚集蛋白聚糖(AGC)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Aggrecan (AGC)

ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

  • 聚集蛋白聚糖(AGC)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 聚集蛋白聚糖(AGC)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 聚集蛋白聚糖(AGC)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB908Po.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测聚集蛋白聚糖(AGC)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将聚集蛋白聚糖(AGC)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的聚集蛋白聚糖(AGC)与连接于固相载体上的抗体结合，然后加入生物素化的聚集蛋白聚糖(AGC)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的聚集蛋白聚糖(AGC)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Sus scrofa; Porcine (Pig，猪) 应用(仅供研究使用，不用于临床诊断！)
SEB908Po 聚集蛋白聚糖(AGC)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source]
Age( dordr). In vitro tenocyte metabolism in aging and oestrogen deficiency [Springer: Source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Stem Cell Research & Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. [Pubmed: 24739658]
Materials Science and Engineering: C Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition [ScienceDirect: S0928493113006656]
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
Macromolecular Bioscience Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration [PubMed: 25787871]
Osteoarthritis and Cartilage Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs [Pubmed:26802547]
Cell Proliferation Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes [Pubmed:27090063]
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731]
Journal of Clinical Medicine Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors [Pubmed: 32392807]
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs []
researchsquare Aggrecan-a new biomarker for acute type A aortic dissection [33990642]
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