血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Angiotensin 1-7 (Ang1-7)

  • 血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CES085Mi.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒对检测血管紧张素1-7(Ang1-7)具有较高的灵敏度。
经检测人、小鼠、大鼠之间有100%的交叉反映。

回收率

分别于定值血清及血浆样本中加入一定量的血管紧张素1-7(Ang1-7)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 84-98 91
EDTA plasma(n=5) 96-103 101
heparin plasma(n=5) 94-105 102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的血管紧张素1-7(Ang1-7),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中血管紧张素1-7(Ang1-7)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-104% 84-104% 86-96% 85-95%
EDTA plasma(n=5) 89-103% 99-105% 91-101% 79-101%
heparin plasma(n=5) 78-98% 90-97% 83-94% 84-92%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将血管紧张素1-7(Ang1-7)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
CPS085Mi21 血管紧张素1-7(Ang1-7)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPS085Mi11 血管紧张素1-7(Ang1-7)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAS085Mi08 血管紧张素1-7(Ang1-7)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAS085Mi22 血管紧张素1-7(Ang1-7)单克隆抗体 WB; IHC; ICC; IP.
MAS085Mi21 血管紧张素1-7(Ang1-7)单克隆抗体 WB; IHC; ICC; IP.
CES085Mi 血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMS085Mi 血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
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Cellular Physiology and Biochemistry Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats [pubmed:28848201]
Frontiers in Physiology Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression [pubmed:29066981]
Molecular Medicine Reports The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction. [pubmed:28656296]
Journal of Clinical Pharmacy and Therapeutics Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites. [pubmed:28895159]
Molecular Medicine Reports Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats [pubmed:28944898]
Open Journal of Endocrine and Metabolic Diseases Paper Submission A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats [2-1980272_83198.htm]
Journal of the American Society of Hypertension Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system [Pubmed:29752040]
Innate Immunity  Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis [Pubmed:29969931]
Evidence-based Complementary and Alternative Medicine  Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313]
European Journal of Obstetrics & Gynecology and Reproductive Biology The impact of ovulation inducing drugs on carcinogenesis []
European Journal of Obstetrics & Gynecology and Reproductive Biology Changes of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia []
Journals of Gerontology Series A-Biological Sciences and Medical Sciences Therapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study. [Pubmed: 31586210]
journal of the european academy of dermatology and venereology Significance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosis [Pubmed: 31746507]
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum … []
Amino Acids Angiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats [33398523]
Autoimmunity The prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis [33910447]
researchsquare Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice []
Peptides Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide [34298021]
BMC Musculoskeletal Disorders Ang-(1–7) protects skeletal muscle function in aged mice [34548056]
J Cardiovasc Pharmacol Resveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy [34232225]
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