抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Anti-Gamma-Aminobutyric Acid Antibody (Anti-gABA)

4-Aminobutyric Acid

  • 抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • AEA900Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测抗γ-氨基丁酸抗体(Anti-gABA),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的抗γ-氨基丁酸抗体(Anti-gABA)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 95-105 101
EDTA plasma(n=5) 84-91 87
heparin plasma(n=5) 80-96 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的抗γ-氨基丁酸抗体(Anti-gABA),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中抗γ-氨基丁酸抗体(Anti-gABA)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 81-93% 94-102% 80-101%
EDTA plasma(n=5) 91-102% 92-99% 80-95% 92-101%
heparin plasma(n=5) 86-93% 92-101% 96-103% 87-103%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板5次;
5. 加TMB底物90µL,37°C孵育10-20分钟;
6. 加终止液50µL,立即450nm读数。

实验原理

将抗原包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的抗γ-氨基丁酸抗体(Anti-gABA)与连接于固相载体上的抗原结合,然后加入HRP标记的二抗,彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗γ-氨基丁酸抗体(Anti-gABA)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA900Ge11 γ-氨基丁酸(gABA)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA900Ge21 γ-氨基丁酸(gABA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA900Ge41 γ-氨基丁酸(gABA)人血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA900Ge01 γ-氨基丁酸(gABA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA900Ge71 γ-氨基丁酸(gABA)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA900Ge81 γ-氨基丁酸(gABA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
CEA900Ge γ-氨基丁酸(gABA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA900Ge 抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
LMA900Ge γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA900Ge11 γ-氨基丁酸(gABA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825]
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604]
Scientific Reports Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension [pubmed:27917928]
Journal of Neuroscience Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894]
Scientific Reports A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme [Pubmed: 30341396]
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Neuroscience Letters Differentiation of human glioblastoma U87 cells into cholinergic neuron [Pubmed: 30928478]
Neurochemical Journal Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism []
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Experimental and Therapeutic Medicine Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy [Pubmed: 33101467]
Exp Anim Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation [33828024]
Neurogenetics Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases [34101060]
Sci Rep Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission [34475508]
J Food Biochem Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies [Pubmed:35102569]
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