抗缪勒管激素(AMH)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Anti-Mullerian Hormone (AMH)

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

  • 抗缪勒管激素(AMH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗缪勒管激素(AMH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗缪勒管激素(AMH)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA228Bo.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测抗缪勒管激素(AMH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的抗缪勒管激素(AMH)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 95-102 99
EDTA plasma(n=5) 86-99 92
heparin plasma(n=5) 97-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的抗缪勒管激素(AMH),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中抗缪勒管激素(AMH)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-97% 88-97% 83-91% 87-103%
EDTA plasma(n=5) 79-96% 92-99% 94-102% 79-104%
heparin plasma(n=5) 96-104% 83-104% 89-103% 98-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将抗缪勒管激素(AMH)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
RPA228Bo01 抗缪勒管激素(AMH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA228Bo01 抗缪勒管激素(AMH)多克隆抗体 WB; IHC; ICC; IP.
MAA228Bo21 抗缪勒管激素(AMH)单克隆抗体 WB; IHC; ICC; IP.
CEA228Bo 抗缪勒管激素(AMH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA228Bo 抗缪勒管激素(AMH)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Journal of Zhejiang University SCIENCE B Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. [PubMed: 23125082]
In Vivo.(Athens,Greece) Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF [Pubmed: 24292596]
Gynecological Endocrinology The effects of isotretinoin on the ovarian reserve of females with acne [Informahealthcare: Source]
Fertility and Sterility Increased expression of anti mullerian hormone and its receptor in endometriosis [Ingentaconnect: Source]
Reproductive BioMedicine Online Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure [Rbmojournal: Source]
Reproductive Biology and Endocrinology The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. [Pubmed: 24341292]
Biomed Res Int. Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats [Pubmed:Pmc4053297]
Fertil Steril. Increased expression of antimüllerian hormone and its receptor in endometriosis [Pubmed:24613539]
The Journal of Maternal-Fetal &amp; Neonatal Medicine Maternal serum anti-Müllerian hormone at 11-13 weeks&#039; gestation in the prediction of preeclampsia [Pubmed:24953352]
J Gynecol Oncol. Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model [Pubmed:25142624]
Reproductive BioMedicine Online The effect of childhood cancer therapy on ovarian reserve and pubertal development [Pubmed:25498597]
Reproductive Sciences Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome [Pubmed:25667201]
Yonsei Medical Journal Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines [PubMed: 26632380]
oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats [PubMed: 26629940]
Reprod Biomed Online Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats [PubMed: 26206282]
PLoS One Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis [PubMed: 26465611]
Toxicol Lett Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood [PubMed: 26657179]
Journal of Assisted Reproduction and Genetics Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage? [Pubmed:27255570]
Anticancer Res. Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer [Pubmed:26976997]
Chemosphere. Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. [pubmed:27567155]
Theriogenology Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. [pubmed:28043339]
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061]
Journal of Assisted Reproduction and Genetics Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. [pubmed:28074436]
Journal Pakistan Medical Association Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice [pubmed:27654725]
Advance Medical Associates Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? [pubmed:27162065]
Yonsei Med J Volume Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. [pubmed:26632380]
Journal of Endocrinological Investigation The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF. [pubmed:27465668]
Journal of the Pakistan Medical Association Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice [pubmed:28955058]
International Journal of Reproduction, Contraception, Obstetrics and Gynecology Can Co-enzyme Q10 improve the chances of conception after the age of 35? [10.18203/2320-1770.ijrcog20172902]
Animal Production Science Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? [10.1071/ANv57n12Ab065]
Reproduction. Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring [pubmed:28687593]
Theriogenology Relationship among circulating anti-Müllerian hormone, insulin like growth factor 1, cadmium and superovulatory response in dairy cows. [pubmed:28708536]
Journal of ovarian research Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo. [pubmed:28814333]
Annals of Rheumatic Diseases  FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study [Suppl_2:728.1.abstract]
Free Radical Biology & Medicine Peroxiredoxin 2 deficiency accelerates age-related ovarian failure through the reactive oxygen species-mediated JNK pathway in mice [Pubmed:29778464]
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625]
Biology of Reproduction Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss [Pubmed:29351580]
Endocrine Connections Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery [Pubmed:30012648]
Reproductive BioMedicine Online Dienogest suppresses the activation of primordial follicles and preserves the primordial follicle stockpile for fertility in mice [Pubmed:29609766]
Journal of Ovarian Research Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian … [Pubmed:29859107]
Animal Reproduction Science Anti-Müllerian hormone and Oestradiol as markers of future reproductive success in juvenile gilts [Pubmed:29859701]
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … [Pubmed: 30606243]
Biochemical and Biophysical Research Communications Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells [Pubmed: 30722991]
Lupus Levels of anti-Müllerian hormone in premenopausal women with the antiphospholipid syndrome and its association with the risk of clinical complications [Pubmed: 30717622]
In Vitro Cellular & Developmental Biology - Animal Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice [Pubmed: 30820812]
Biomedicine & Pharmacotherapy Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve []
Reproductive Biology and Endocrinology Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … [Pubmed: 31311554]
Oxidative Medicine and Cellular Longevity Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure []
Acta Obstet Gynecol Scand The impact of uterine artery embolization on ovarian reserve: a systematic review and meta‐analysis [Pubmed: 31370100]
Reproductive Sciences Uterine Cells Improved Ovarian Function in a Murine Model of Ovarian Insufficiency [Pubmed: 31530098]
Animals Serum Concentrations of AMH and E2 and Ovarian and Uterine Traits in Gilts [Pubmed: 31619004]
Journal Of Pakistan Medical Association The effect of acupuncture on anti-mullerian hormone and assisted reproduction outcome in Polycystic Ovary Syndrome patients undergoing in vitro fertilization … [Pubmed: 31603867]
In Vitro Cellular & Developmental Biology Basal characterization and in vitro differentiation of putative stem cells derived from the adult mouse ovary [Pubmed: 31900800]
J Assist Reprod Genet The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage [Pubmed: 32020412]
Biology (Basel). Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker? [Pubmed: 32290279]
Eur J Breast Health The Effect of Systemic Chemotherapy on Ovarian Function: A Prospective Clinical Trial [Pubmed: 32656517]
Heliyon Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … [Pubmed: 33005806]
CYTOMETRY PART A After cyclophosphamide exposure, granulosa cells recover their anti©\M¨¹llerian hormone©\producing ability but not their numbers [33342073]
BIOLOGY OF REPRODUCTION Igf3 is essential for ovary differentiation in zebrafish [33276384]
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
Stem Cells Int Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … [34733331]
Sci Total Environ Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression [Pubmed:35276145]
Hum Reprod Update Anti-Müllerian hormone as a marker of ovarian reserve and premature ovarian insufficiency in children and women with cancer: a systematic review [Pubmed:35199161]
Horm Metab Res Anti-Mullerian Hormone Levels Increase After Bariatric Surgery in Obese Female Patients With and Without Polycystic Ovary Syndrome [Pubmed:35276745]
American Journal of Translational Research The effects of vasopressin injection technique on ovarian reserve in laparoscopic cystectomy of bilateral ovarian endometrioma: a retrospective cohort study [Pubmed:35559399]
Stem Cell Research & Therapy G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and … []
Research Square Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency []
Fundamental Research Long-term amelioration of an early-onset familial atrial fibrillation model with AAV-mediated in vivo gene therapy []
留言咨询