骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)

BMP2A; BMP-2A; Hemochromatosis Modifier

  • 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA013Ca.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测骨成型蛋白2(BMP2),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的骨成型蛋白2(BMP2)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-94 79
EDTA plasma(n=5) 80-93 86
heparin plasma(n=5) 94-101 98

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的骨成型蛋白2(BMP2),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中骨成型蛋白2(BMP2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 89-101% 97-105% 93-101% 83-95%
EDTA plasma(n=5) 83-97% 97-104% 96-105% 84-99%
heparin plasma(n=5) 86-98% 92-101% 81-102% 86-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将骨成型蛋白2(BMP2)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的骨成型蛋白2(BMP2)与连接于固相载体上的抗体结合,然后加入生物素化的骨成型蛋白2(BMP2)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的骨成型蛋白2(BMP2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Canis familiaris; Canine (Dog,犬) 应用(仅供研究使用,不用于临床诊断!)
RPA013Ca01 骨成型蛋白2(BMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA013Ca02 骨成型蛋白2(BMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA013Ca01 骨成型蛋白2(BMP2)多克隆抗体 WB; IHC; ICC; IP.
PAA013Ca02 骨成型蛋白2(BMP2)多克隆抗体 WB; IHC; ICC; IP.
LAA013Ca71 骨成型蛋白2(BMP2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA013Ca 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA013Ca 骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice [Wiley: Source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Medical Journal of Chinese People&#039;s Liberation Army Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion [Source]
PLOS ONE PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling [Pubmed:25470749]
Eur J Pharmacol Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy [PubMed: 25934570]
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861]
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway [PubMed: 26681207]
International Journal of Nanomedicine Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis [Pubmed:27042064]
Research report EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO [files:p013-022]
Gene  Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE [Pubmed:27196063]
Journal of Materials Chemistry B Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair [DOI:10.1039/C7TB00640C]
Scientific Reports 3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering. [pubmed:29042614]
Journal of Applied Biomaterials & Functional Materials Demineralized dentin and enamel matrices as suitable substrates for bone regeneration [pubmed:28731486]
Journal of Clinical Periodontology  Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908]
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457]
Journal of Applied Biomaterials & Functional Materials BMP-2 and type I collagen preservation in human deciduous teeth after demineralization [Pubmed:30045659]
Applied Surface Science Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction []
Materials Science and Engineering: C Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration []
Biomaterials Science Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration []
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats [Pubmed: 32004919]
Journal of Industrial and Engineering Chemistry Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration []
BMC Musculoskeletal Disorders Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse []
Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739]
Tissue Eng Regen Med Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro [34302695]
Toxicon The WNT/β-catenin signalling pathway induces chondrocyte apoptosis in the cartilage injury caused by T-2 toxin in rats [34742779]
ACS Biomater Sci Eng Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum [34472855]
Phytomedicine Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane [Pubmed:35278899]
留言咨询