促甲状腺素(TSH)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Thyroid Stimulating Hormone (TSH)
Thyrotropin
- 编号CEA463Ca
- 物种Canis familiaris; Canine (Dog，犬) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围24-15,000pg/mL
- 灵敏度最小可检测剂量小于等于11pg/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3024 ¥ 4320 ¥ 19440 ¥ 36720 ¥ 302400
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测促甲状腺素(TSH)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的促甲状腺素(TSH)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 83-94 | 87 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 79-103 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的促甲状腺素(TSH)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中促甲状腺素(TSH)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-104% | 94-101% | 87-94% | 95-103% |
| EDTA plasma(n=5) | 78-93% | 80-104% | 95-103% | 89-97% |
| heparin plasma(n=5) | 85-94% | 98-105% | 82-91% | 97-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将促甲状腺素(TSH)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Canis familiaris; Canine (Dog，犬) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA463Ca | 促甲状腺素(TSH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA463Ca | 促甲状腺素(TSH)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA463Ca | 促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA463Ca11 | 促甲状腺素(TSH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Blood | Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID [Pubmed: 22184407] |
| Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies [Lifesciencesite: Source] |
| Thyroid | Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function [Pubmed: 23819433] |
| Journal of Functional Foods | Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar rats [Sciencedirect:S1756464615000894] |
| J Endocrinol. | Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis [Pubmed:24928937] |
| journal of lipid research | Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver [PubMed: 25713102] |
| Int J Clin Exp Med | Lead exposure causes thyroid abnormalities in diabetic rats [PubMed: 26221254] |
| Jubilant Drax Image | Perinatal Iron Deficiency-Induced Hypothyroxinemia Impairs Early Brain Development Regardless of Normal Iron Levels in the Neonatal Brain [Pubmed:27231981] |
| PLoS One. | The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats [Pubmed:27043135] |
| journal of bone and mineral research | Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis. [pubmed:27589370] |
| Toxicological Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
| Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders [handle:123456789] |
| International Journal of Peptide Research and Therapeutics | Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteins [article:10.1007] |
| Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats [10.4314/tjpr.v16i5.9] |
| 30 | The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. [pubmed:28546511] |
| NITRIC OXIDE-BIOLOGY AND CHEMISTRY | Voluntary exercise training restores anticontractile effect of NO in coronary arteries of adult rats with antenatal/early postnatal hypothyroidism [Pubmed:29307633] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
| Neuroscience Letter | Central irisin administration suppresses thyroid hormone production but increases energy consumption in rats [Pubmed:29574218] |
| Journal of Neuroinflammation | Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice [Pubmed: 30373627] |
| Cell Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
| Journal of Diabetes Research | The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice [] |
| Endocrine | Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity [Pubmed: 31056723] |
| Oxidative Medicine and Cellular Longevity | Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of … [] |
| Reproductive Biology and Endocrinology | Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice [Pubmed: 31729993] |
| Gli | Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice [Pubmed: 32173924] |
| Clinical Immunology | The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice [Pubmed: 31982645] |
| Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis [Pubmed: 33177714] |
| Thyroid | Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation [Pubmed: 32924834] |
| Thyroid | Hashimoto's thyroiditis induces hippocampus-dependent cognitive alterations by impairing astrocytes in euthyroid mice [Pubmed: 32907517] |
| Int Immunopharmacol | Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy [Pubmed: 32442899] |
| Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa … [] |
| Comparaison des marqueurs hormonaux et histomorphométriques chez la souris un modèle d'hypothyroïdie après 28 jours de traitement au PTU par voie alimentaire. [] | |
| Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation [33433934] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart [33375430] |
| Biomedicines | Role of Kallikrein 7 in Body Weight and Fat Mass Regulation. Biomedicines 2021, 9, 131 [33572949] |
| Mol Neurobiol | Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75 NTR Signal Pathway [33966253] |
| Clin Epigenetics | Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism [34092241] |
| Pharmaceutics | Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays [] |
| BRITISH JOURNAL OF NUTRITION | Effects of iodine excess on serum thyrotropin-releasing hormone levels and type 2 deiodinase in the hypothalamus of Wistar rats [34250878] |
| Gene Therapy | Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR2A vector [34599290] |
| NatCommun | Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis [34876572] |
| Int J Mol Sci | The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism [Pubmed:35163456] |
| Molecular Biology Reports | Effects of meteorin-like hormone on endocrine function of hypothalamo-hypophysial system and peripheral uncoupling proteins in rats [Pubmed:35332411] |
留言咨询
