和肽素(CPP)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Copeptin (CPP)
C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin
- 编号CEA365Po
- 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围12.35-1,000pg/mL
- 灵敏度最小可检测剂量小于等于5.45pg/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测和肽素(CPP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的和肽素(CPP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 96-104 | 99 |
| EDTA plasma(n=5) | 92-105 | 101 |
| heparin plasma(n=5) | 79-88 | 80 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的和肽素(CPP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中和肽素(CPP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-95% | 78-92% | 98-105% | 93-101% |
| EDTA plasma(n=5) | 98-105% | 85-101% | 93-105% | 87-95% |
| heparin plasma(n=5) | 96-104% | 89-96% | 79-98% | 79-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将和肽素(CPP)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA365Po | 和肽素(CPP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Med-Science. | Copeptin: May it be a novel biomarker for insulin theraphy in diabetes? An animal study. [Ejmanager: Source] |
| Psychiatry and Clinical Neurosciences | Serum copeptin in children exposed to maltreatment [Pubmed:27278269] |
| United States Patent Application | Methods for Diagnosis and Treatment of Concussion or Brain Injury [2016:0103140] |
| J Physiol | Role of enteroendocrine L‐cells in arginine vasopressin‐mediated inhibition of colonic anion secretion [Pubmed:27037495] |
| International Journal of Clinical and Experimental Medicine | Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … [files:ijcem0031358.pdf] |
| Front Physiol | Orthostatic Challenge Shifts the Hemostatic System of Patients Recovered from Stroke toward Hypercoagulability. [pubmed:28223937] |
| Kidney Blood Pressure Research | N-Terminal Prohormone of Brain Natriuretic Peptide but not C-Terminal Pre-Pro Vasopressin (Copeptin) Level is Associated with the Response to Antihypertensive … [pubmed:29190613] |
| Hindawi Publishing Corporation | Early Prediction of Preeclampsia [journals:297397] |
| Journal of Urology | Evaluation of Urinary Aquaporin 2 and Plasma Copeptin as Biomarkers of Effectiveness of Desmopressin Acetate for the Treatment of Monosymptomatic Nocturnal Enuresis. [pubmed:28457803] |
| American Journal of Physiology-Renal Physiology | Experimental Heat Stress Nephropathy and Liver Injury are Improved by Allopurinol [Pubmed:29667911] |
| American Journal of Physiology-Regulatory Integrative and Comparative Physiology | Angiotensin AT Receptors Expressed in Vasopressin-Producing Cells of the Supraoptic Nucleus Contribute to the Osmotic Control of Vasopressin [Pubmed:29364700] |
| BMC Nephrology | Rehydration with fructose worsens dehydration-induced renal damage [Pubmed:30005632] |
| Experimental and Therapeutic Medicine | Copeptin level in the early prediction of cardiorenal syndrome in rats [Pubmed:30112047] |
| 中国病理生理杂志 | 和肽素水平对大鼠心肾综合征的早期预测价值 [filename:ZBLS201802013] |
| Hypertension | Abstract P311: Static Urine Osmolality With Elevated First Trimester Urine Copeptin in Human Preeclampsia [] |
| Journal of Pediatric Urology | Assessment of serum levels of copeptin and corticotropin-releasing factor in children with monosymptomatic and nonmonosymptomatic nocturnal enuresis [] |
| experimental physiology | Cognitive performance is associated with cerebral oxygenation and peripheral oxygen saturation, but not plasma catecholamines, during graded normobaric hypoxia [] |
| international urology and nephrology | Dialysate copeptin and peritoneal transport in incident peritoneal dialysis patients [Pubmed: 31187425] |
| American Journal of Physiology-renal Physiology | The contribution of collecting duct NOS1 to the concentrating mechanisms in male and female mice [Pubmed: 31241990] |
| american journal of physiology-renal physiology | The increase of core temperature affected the progression of kidney injury by repeated heat stress exposure [Pubmed: 31390229] |
| Scientific Reports | Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion [Pubmed: 31863073] |
| BMC Nephrology | Copeptin is independently associated with vascular calcification in chronic kidney disease stage 5 [Pubmed: 32033584] |
| Archives of Biochemistry and Biophysics | Micro RNA 146a gene variant/TNF-α/IL-6/IL-1 β; A cross-link axis inbetween oxidative stress, endothelial dysfunction and neuro-inflammation in acute ischemic stroke … [Pubmed: 31738890] |
| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789] |
| Biomolecules . | Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study [Pubmed: 32549327] |
| Arch Med Res | Relation of Copeptin with Diabetic and Renal Function Markers Among Patients with Diabetes Mellitus Progressing Towards Diabetic Nephropathy [Pubmed: 32505416] |
| 河北医科大学学报 | 重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响 [] |
| Scientific reports | Validity of different copeptin assays in the differential diagnosis of the polyuria-polydipsia syndrome [33980941] |
| International Journal of Women¡¯s Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? [] |
| Dicle T?p Dergisi | Copeptin levels are more elevated and associated with cardiovascular events in the unity of chronic ischemic heart and chronic kidney diseases [] |
| High serum copeptin may be a marker of an increased carotid intima-media thickness in asymptomatic patients with type 1 diabetes. [] | |
| Nutrients | The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose [] |
| Acta Physiol (Oxf) | Dietary K+ acts as a genuine diuretic [Pubmed:34984847] |
| Anal Chim Acta | Spectrally multiplexed assay using gap enhanced nanoparticle for detection of a myocardial infarction biomarker panel [Pubmed:35190126] |
| J Cell Physiol | Arcuate NPY is involved in salt‐induced hypertension via modulation of paraventricular vasopressin and brain‐derived neurotrophic factor [Pubmed:35312067] |
| Children | Copeptin Concentrations in Plasma of Healthy Neonates in Relation to Water–Electrolyte Homeostasis in the Early Adaptation Period [Pubmed:35327814] |
