生长激素(GH)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Growth Hormone (GH)

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

  • 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA044Eq.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测生长激素(GH)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将生长激素(GH)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。

相关产品

编号 适用物种：Equus caballus; Equine (Horse，马) 应用(仅供研究使用，不用于临床诊断！)
RPA044Eq01 生长激素(GH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA044Eq01 生长激素(GH)多克隆抗体 WB; IHC; ICC; IP.
MAA044Eq21 生长激素(GH)单克隆抗体 WB; IHC; ICC; IP.
CEA044Eq 生长激素(GH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA044Eq 生长激素(GH)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Neuroscience Letters The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats [PubMed: 19765635]
Cellular Reprogramming Changes in the Somatotrophic Axis in Genetically Identical Dogs [PubMed: 21740269]
Clinical Cancer Research The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status [PubMed: 21642381]
General and Comparative Endocrinology Growth hormone expression and neuroprotective activity in a quail neural retina cell line [ScienceDirect: S0016648009002457]
Nutrition and Metabolism Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis [Nutrition&Metabolism: source]
Veterinary Recprd Case Report Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism [Bmj: Source]
Journal of the American Animal Hospital Association GH Deficiency in a Dalmatian Puppy with Megaesophagus [Pubmed: 24051259]
Biotechnology and Bioprocess Engineering Functional expression of bovine growth hormone gene in Pleurotus eryngii [Springer: Source]
Buletin Veteriner Udayana Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali [Unud:Source]
Veterinary World Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows [Veterinaryworld:Source]
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831]
Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks [Pubmed:26948666]
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87]
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87]
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
Jurnal Riset Akuakultur TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA [1329]
Trop Anim Health Prod Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloes [pubmed:28785907]
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet [article:10.1007]
Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken [10.159:1806-9061-2016-0285]
畜牧兽医学报 单色绿光对鸡胚褪黑激素合成的影响 [DOI: 10.11843/j.issn.0366-6964.2017.05.019]
Obesity Surgery Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice [pubmed:28853013]
JURNAL VETERINER Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS) [10.19087/jveteriner.2017.18.2.226]
Veterinary World. Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia. [pubmed:29184372]
Biochemistry, Genetics and Molecular Biology Effects of Dietary Lysine Levels on the Plasma Concentrations of Growth‐Related Hormones in Late‐Stage Finishing Pigs [10.5772/intechopen.68545]
Cellular Physiology and Biochemistry Decreased Hepatic Lactotransferrin Induces Hepatic Steatosis in Chronic Non-Alcoholic Fatty Liver Disease Model [Pubmed:29975946]
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens [Pubmed:29373588]
Pakistan Journal of Zoology Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. [:]
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008]
International Journal of Current Microbiology and Applied Sciences Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons []
Photochemistry and Photobiology Melatonin Receptor Mel1b‐and Mel1c‐mediated Green Light Induced the Secretion of Growth Hormone in Anterior Pituitary of Chicks [Pubmed: 31111492]
Journal of Animal Science Dietary lysine affects amino acid metabolism and growth performance, which may not involve the GH/IGF-1 axis, in young growing pigs [Pubmed: 31922564]
SAUDI MEDICAL JOURNAL The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additive [Pubmed: 31287125]
Frontiers in Endocrinology RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034]
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION Isolated growth hormone deficiency in a Chihuahua with a GH1 mutation [Pubmed: 32646299]
Scientific Reports Controlled elevated temperatures during early-mid gestation cause placental insufficiency and implications for fetal growth in pregnant pigs [33244103]
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling [33492754]
animals Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes [34359233]
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