核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Nuclear Factor Kappa B (NFkB)

NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit

  • 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB824Ga.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测核因子κB(NFkB)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将核因子κB(NFkB)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的核因子κB(NFkB)与连接于固相载体上的抗体结合，然后加入生物素化的核因子κB(NFkB)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的核因子κB(NFkB)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Chicken (Gallus，鸡) 应用(仅供研究使用，不用于临床诊断！)
RPB824Ga01 核因子κB(NFkB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB824Ga01 核因子κB(NFkB)多克隆抗体 WB; IHC; ICC; IP.
MAB824Ga21 核因子κB(NFkB)单克隆抗体 WB; IHC; ICC; IP.
SEB824Ga 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB824Ga 核因子κB(NFkB)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Reproductice Science Surgical Removal of Endometrioma Decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) Expression in the Eutopic Endometrium During the Implantation Window [Sagepub: Source]
International Immunopharmacology Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitis [PubMed: 21642017]
PLoS One New Method to Isolate and Culture Rat Kupffer Cells [PubMed: PMC3743898]
World Journal of Gastroenterology Long-term aspirin pretreatment in the prevention of cerulein-induced acute pancreatitis in rats [PubMed: PMC3660814]
Experimental and Therapeutic Medicine Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB [PubMed: PMC3570159]
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434]
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
BBA Clin Long-term warfarin therapy and biomarkers for osteoporosis and atherosclerosis [PubMed: 26674156]
J Clin Diagn Res Serological Analysis of Alpha-synuclein and NF-κB in Parkinson’s Disease Patients [PubMed: 26155470]
Eur J Obstet Gynecol Reprod Biol Hysteroscopic polypectomy decreases NF-κB1 expression in the mid-secretory endometrium of women with endometrial polyp [PubMed: 25898371]
Asian Pacific Journal of Cancer Prevention Plasma nuclear factor kappa B and serum peroxiredoxin 3 in early diagnosis of hepatocellular carcinoma. [PubMed: 25743848]
Acta Biochim Pol Preventive use of Lactobacillus plantarum LS/07 and inulin to relieve symptoms of acute colitis. [PubMed: 26345092]
Acta Veterinaria Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute Colitis [View: J]
Neurochem Res Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436]
Transplant Proc L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients [PubMed: 26518955]
Journal of Food Science  Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine [Pubmed:27061742]
IJBPAS THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS [publication:298838934]
Basic & Clinical Pharmacology & Toxicology Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway [doi:10.1111]
Peptides. Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064]
british journal of pharmacology Forskolin, a hedgehog signalling inhibitor, attenuates carbon tetrachloride-induced liver fibrosis in rats. [pubmed:27590029]
european journal of pharmacology Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041]
european journal of pharmacology S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948]
Psychopharmacology (Berl) Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. [pubmed:28303372]
biomedicine & pharmacotherapy Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883]
biomedicine & pharmacotherapy Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation. [pubmed:28033582]
Toxicology and Applied Pharmacology Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454]
Microbial Immunology Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota [pubmed:28713364]
Journal of Biomechanics The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis [pubmed:29137729]
European Journal of Pharmacology In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesis [pubmed:29126792]
Frontiers in Pharmacology Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus. [pubmed:28553224]
International Journal of Medicinal Mushrooms Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus. [pubmed:29256845]
Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403]
International Immunopharmacology Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms. [pubmed:29190543]
Food and Chemical Toxicology Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088]
Neurotoxicity Research Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells [Pubmed:29869772]
European Journal of Pharmacology  Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat [Pubmed:29518393]
Experimental biology and medicine (Maywood) Shock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse model [Pubmed:29984607]
Acta Veterinaria Belgrade Anti-inflammatory potential of Lactobacillus plantarum LS/07 in acute colitis in rats [10.2478:acve-2018-0005]
Taiwanese Journal of Obstetrics & Gynecology HMGB1-RAGE signaling pathway in pPROM [Pubmed:29673663]
Acta Veterinaria Brno Improvement of colitis by LS/07 and inulin [10.2754:avb201786040399]
Drug Discoveries & Therapeutics The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4 [Pubmed:29760339]
Life Sciences Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096]
Life Sciences Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner [Pubmed: 30195035]
Journal of Traditional Chinese Medical Sciences Effect of Dachaihu Decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet []
Naunyn-Schmiedeberg's archives of pharmacology Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2 [Pubmed: 30474696]
Archives of Physiology and Biochemistry Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216]
Journal of Functional Foods Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments []
Scientific Reports Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis [Pubmed: 30651572]
Journal of Pain Researc Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain [Pubmed: 30881100]
Antioxidants Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian … [Pubmed: 31600920]
journal of biochemical and molecular toxicology Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells [Pubmed: 31916655]
Life Sci New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signaling [Pubmed: 32209424]
European Review for Medical and Pharmacological Sciences Effects of lncRNA gm4419 on rats with hypertensive cerebral atherosclerosis through NF-κB pathway [Pubmed: 31858567]
J Ethnopharmacol Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea [Pubmed: 33217518]
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337]
LIFE SCIENCES Effects of combined treatment with Indomethacin and Juglone on AOM/DSS induced colon carcinogenesis in Balb/c mice: Roles of inflammation and … [Pubmed: 33148421]
JOURNAL OF ETHNOPHARMACOLOGY Morroniside attenuates apoptosis and pyroptosis of chondrocytes and ameliorates osteoarthritic development by inhibiting NF-κB signaling [Pubmed: 33022338]
Journal of Cosmetic Dermatology Examination of androgenetic alopecia with serum biomarkers [Pubmed: 32969583]
NEUROPSYCHOBIOLOGY Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms [Pubmed: 32526752]
journal of ethnopharmacology UPLC-Orbitrap HRMS metabolic profiling of Cymbopogon citratus cultivated in Egypt; neuroprotective effect against AlCl3-induced neurotoxicity in rats [Pubmed: 32376365]
BIOMEDICINE & PHARMACOTHERAPY Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939]
Journal of Diabetes Research Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus [Pubmed: 33123597]
Folia Biologica Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats []
TOXICOLOGY IN VITRO Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells [33675893]
Hum Exp Toxicol Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats [33882726]
J Food Biochem Masseter muscle and gingival tissue inflammatory response following treatment with high©\fructose corn syrup in rats: Anti©\inflammatory and antioxidant effects of kefir [33864286]
Open Access Macedonian Journal of Medical Sciences The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation []
Eur Rev Med Pharmacol Sci Vitamin D supplementation inhibits NF-kß signaling pathway in lean and obese women with PCOS [Pubmed:35731068]
BMC Nephrol Effect of subacute poisoning with lambdacyhalothrin on vascular endothelial growth factor 2 receptor in mice kidneys [Pubmed:35643467]
Antioxidants Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … [Pubmed:35204140]
Journal of Pharmaceutical Research International Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid … []
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