促肾上腺皮质激素(ACTH)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Adrenocorticotropic Hormone (ACTH)

Corticotropin; Adrenocorticotropin

  • 促肾上腺皮质激素(ACTH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 促肾上腺皮质激素(ACTH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 促肾上腺皮质激素(ACTH)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA836Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测促肾上腺皮质激素(ACTH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的促肾上腺皮质激素(ACTH)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-105 91
EDTA plasma(n=5) 88-97 93
heparin plasma(n=5) 97-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的促肾上腺皮质激素(ACTH),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中促肾上腺皮质激素(ACTH)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 81-103% 79-89% 78-97%
EDTA plasma(n=5) 80-96% 90-99% 80-101% 90-103%
heparin plasma(n=5) 78-97% 86-98% 78-103% 80-91%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将促肾上腺皮质激素(ACTH)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
CPA836Hu11 促肾上腺皮质激素(ACTH)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA836Hu21 促肾上腺皮质激素(ACTH)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA836Hu08 促肾上腺皮质激素(ACTH)多克隆抗体 WB; IHC; ICC; IP.
LAA836Hu81 促肾上腺皮质激素(ACTH)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA836Hu21 促肾上腺皮质激素(ACTH)单克隆抗体 WB; IHC; ICC; IP.
MAA836Hu28 促肾上腺皮质激素(ACTH)单克隆抗体 WB; IHC; ICC; IP.
CEA836Hu 促肾上腺皮质激素(ACTH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA836Hu 促肾上腺皮质激素(ACTH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA836Hu11 促肾上腺皮质激素(ACTH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Respir Res. Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. [PubMed: 20403205]
Toxicology Letters Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345]
Mol Med Rep. Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. [Pubmed:24682498]
Peptides Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411]
Journal of Animal and Feed Sciences Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages [Researchgate:Source]
International Journal of Immunopathology & Pharmacology Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638]
Cell Tissue Res CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release [PubMed: 26572542]
Neuroscience Letters Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866]
Neural Regeneration Research Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. [Pubmed:27212922]
Environmental Science and Pollution Research Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway [Pubmed:27102619]
Sci Rep. Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland [Pubmed:27109116]
Sci Rep. High activity of the stress promoter contributes to susceptibility to stress in the tree shrew [Pubmed:27125313]
brain research bulletin Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. [pubmed:28161195]
中国临床药理学与治疗学 肉蔻五味丸抗抑郁作用及机制研究 [9787]
Neuroscience Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093]
Toxicology Mixture effects of azole fungicides on the adrenal gland in a broad dose range [pubmed:28454766]
Biomedicine & Pharmacotherapy Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway [pubmed:28892788]
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